Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules.
MLP29
cell crosstalk
exosomes
extracellular vesicles (EVs)
fibroblast
immune response
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
10
2020
accepted:
07
12
2020
entrez:
29
1
2021
pubmed:
30
1
2021
medline:
30
1
2021
Statut:
epublish
Résumé
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
Identifiants
pubmed: 33511119
doi: 10.3389/fcell.2020.613583
pmc: PMC7835421
doi:
Types de publication
Journal Article
Langues
eng
Pagination
613583Informations de copyright
Copyright © 2021 Royo, Azkargorta, Lavin, Clos-Garcia, Cortazar, Gonzalez-Lopez, Barcena, del Portillo, Yáñez-Mó, Marcilla, Borras, Peinado, Guerrero, Váles-Gómez, Cereijo, Sardon, Aransay, Elortza and Falcon-Perez.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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