Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA.
COVID-19
Coronavirus
Diagnostics
Infectious disease
Laboratory diagnostics
Molecular testing
Real-time PCR
SARS-CoV-2
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
04
10
2020
accepted:
17
12
2020
pubmed:
30
1
2021
medline:
28
5
2021
entrez:
29
1
2021
Statut:
ppublish
Résumé
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.
Identifiants
pubmed: 33512617
doi: 10.1007/s10096-021-04159-9
pii: 10.1007/s10096-021-04159-9
pmc: PMC7845288
doi:
Substances chimiques
RNA, Viral
0
Reagent Kits, Diagnostic
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1303-1308Investigateurs
Armin Baiker
(A)
Bernadett Bartha-Dima
(B)
Katja Bengs
(K)
Anja Berger
(A)
Kerstin Boll
(K)
Anja Carl
(A)
Jürgen Christian
(J)
Alexandra Dangel
(A)
Juliana Drdlicek
(J)
David Eisenberger
(D)
Volker Fingerle
(V)
Lars Gerdes
(L)
Ottmar Goerlich
(O)
Patrick Gürtler
(P)
Sabrina Hepner
(S)
Bernhard Hobmaier
(B)
Christine Hupfer
(C)
Regina Konrad
(R)
Sandra Lampl
(S)
Bernhard Liebl
(B)
Gaia Lupoli
(G)
Gabriele Margos
(G)
Roswitha Müller
(R)
Silke Nickel
(S)
Mercy Okeyo
(M)
Melanie Pavlovic
(M)
Sven Pecoraro
(S)
Isabel Sahm
(I)
Melanie Schauer
(M)
Anika Schülein
(A)
Eva-Maria Schürmann
(EM)
Gesine Schulze
(G)
Nelly Scuda
(N)
Stefanie Singer
(S)
Thorsten Stellberger
(T)
Bianca Treis
(B)
Christian Tuschak
(C)
Pia Zimmermann
(P)
Références
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doi: 10.2807/1560-7917.ES.2020.25.9.2000173
pmcid: 7068163
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doi: 10.1016/j.antiviral.2018.07.017
Ren LL, Wang YM, Wu ZQ, Xiang ZC, Guo L, Xu T et al (2020) Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study. Chin Med J 133:1015–1024
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van Kasteren PB, van der Veer B, van den Brink S, Wijsman L, de Jonge J, van den Brandt A, et al (2020) Comparison of seven commercial RT-PCR diagnostic kits for COVID-19. J Clin Virol 128:104412