Uptake of circulating extracellular vesicles from rectal cancer patients and differential responses by human monocyte cultures.
Case-Control Studies
Cell Culture Techniques
Cell Line, Tumor
Cells, Cultured
Chromatography, Gel
Cytokines
/ genetics
Extracellular Vesicles
/ immunology
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Intestinal Polyps
/ immunology
Monocytes
/ cytology
Rectal Neoplasms
/ blood
colorectal cancer
extracellular vesicles
inflammation
monocytes
tumor microenvironment
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
25
09
2020
revised:
13
01
2021
accepted:
26
01
2021
pubmed:
30
1
2021
medline:
28
12
2021
entrez:
29
1
2021
Statut:
ppublish
Résumé
Extracellular vesicles (EVs) released by tumor cells can directly or indirectly modulate the phenotype and function of the immune cells of the microenvironment locally or at distant sites. The uptake of circulating EVs and the responses by human monocytes in vitro may provide new insights into the underlying biology of the invasive and metastatic processes in cancer. Although a mixed population of vesicles is obtained with most isolation techniques, we predominantly isolated exosomes (small EVs) and microvesicles (medium EVs) from the SW480 colorectal cancer cell line (established from a primary adenocarcinoma of the colon) by sequential centrifugation and ultrafiltration, and plasma EVs were prepared from 22 patients with rectal adenoma polyps or invasive adenocarcinoma by size-exclusion chromatography. The EVs were thoroughly characterized. The uptake of SW480 EVs was analyzed, and small SW480 EVs were observed to be more potent than medium SW480 EVs in inducing monocyte secretion of cytokines. The plasma EVs were also internalized by monocytes; however, their cytokine-releasing potency was lower than that of the cell line-derived vesicles. The transcriptional changes in the monocytes highlighted differences between adenoma and adenocarcinoma patient EVs in their ability to regulate biological functions, whereas the most intriguing changes were found in monocytes receiving EVs from patients with metastatic compared with localized cancer.
Identifiants
pubmed: 33512765
doi: 10.1002/2211-5463.13098
pmc: PMC7931235
doi:
Substances chimiques
Cytokines
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
724-740Informations de copyright
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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