HM-chromanone, a component of Portulaca oleracea L., stimulates glucose uptake and glycogen synthesis in skeletal muscle cell.
Animals
Calcium-Calmodulin-Dependent Protein Kinase Kinase
/ metabolism
Cells, Cultured
Deoxyglucose
/ metabolism
Flavonoids
/ pharmacology
Glucose
/ metabolism
Glycogen
/ biosynthesis
Glycogen Synthase Kinase 3
/ metabolism
Muscle, Skeletal
/ cytology
Phosphatidylinositol 3-Kinases
/ metabolism
Phosphorylation
/ drug effects
Portulaca
/ chemistry
Proto-Oncogene Proteins c-akt
/ metabolism
Rats
(E)-5-hydroxy-7-methoxy-3-(2′-hydroxybenzyl)-4-chromanone
L6 skeletal muscle cells
Portulaca oleracea L
glucose uptake
insulin sensitivity
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
04
08
2020
revised:
03
11
2020
accepted:
14
01
2021
pubmed:
30
1
2021
medline:
12
5
2021
entrez:
29
1
2021
Statut:
ppublish
Résumé
Diabetes mellitus is a chronic metabolic disease characterized by increased blood glucose levels. In order to lower blood glucose, it is important to stimulate glucose uptake and glycogen synthesis in the muscle. (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone (HM-chromanone), a constituent isolated from Portulaca oleracea L., exhibits anti-diabetic effects; however, its mechanisms are not yet clearly understood on glucose uptake and glycogen synthesis in muscle cells. In the present study, we examined the effects of HM-chromanone on glucose uptake into L6 skeletal muscle cells and elucidated the underlying mechanisms. The effects of HM-chromanone on glucose uptake into L6 skeletal muscle cells were assessed by 2-Deoxyglucose uptake assay. Western blot analysis was carried out to elucidate the underlying molecular mechanisms. We found that HM-chromanone promoted glucose uptake into L6 skeletal muscle cells in a dose-dependent manner. Moreover, HM-chromanone induced the phosphorylation of IRS-1 The results of this study indicate that HM-chromanone stimulates glucose uptake through the activation of the PI3K/AKT and CaMKKβ-AMPK pathways and glycogen synthesis via the GSK3 α/β pathway in L6 skeletal muscle cells.
Sections du résumé
BACKGROUND
BACKGROUND
Diabetes mellitus is a chronic metabolic disease characterized by increased blood glucose levels. In order to lower blood glucose, it is important to stimulate glucose uptake and glycogen synthesis in the muscle. (E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone (HM-chromanone), a constituent isolated from Portulaca oleracea L., exhibits anti-diabetic effects; however, its mechanisms are not yet clearly understood on glucose uptake and glycogen synthesis in muscle cells.
PURPOSE
OBJECTIVE
In the present study, we examined the effects of HM-chromanone on glucose uptake into L6 skeletal muscle cells and elucidated the underlying mechanisms.
METHODS
METHODS
The effects of HM-chromanone on glucose uptake into L6 skeletal muscle cells were assessed by 2-Deoxyglucose uptake assay. Western blot analysis was carried out to elucidate the underlying molecular mechanisms.
RESULTS
RESULTS
We found that HM-chromanone promoted glucose uptake into L6 skeletal muscle cells in a dose-dependent manner. Moreover, HM-chromanone induced the phosphorylation of IRS-1
CONCLUSION
CONCLUSIONS
The results of this study indicate that HM-chromanone stimulates glucose uptake through the activation of the PI3K/AKT and CaMKKβ-AMPK pathways and glycogen synthesis via the GSK3 α/β pathway in L6 skeletal muscle cells.
Identifiants
pubmed: 33513558
pii: S0944-7113(21)00015-5
doi: 10.1016/j.phymed.2021.153473
pii:
doi:
Substances chimiques
(E)-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone
0
Flavonoids
0
Glycogen
9005-79-2
Deoxyglucose
9G2MP84A8W
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Calcium-Calmodulin-Dependent Protein Kinase Kinase
EC 2.7.11.17
Glycogen Synthase Kinase 3
EC 2.7.11.26
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
153473Informations de copyright
Copyright © 2021. Published by Elsevier GmbH.