Diagnostic Value of Detecting Feline Coronavirus RNA and Spike Gene Mutations in Cerebrospinal Fluid to Confirm Feline Infectious Peritonitis.
Animals
Case-Control Studies
Cats
Coronavirus, Feline
/ genetics
Feline Infectious Peritonitis
/ cerebrospinal fluid
Female
Male
Molecular Diagnostic Techniques
/ veterinary
Mutation
Prospective Studies
RNA, Viral
/ cerebrospinal fluid
Real-Time Polymerase Chain Reaction
/ veterinary
Sensitivity and Specificity
Spike Glycoprotein, Coronavirus
/ genetics
FCoV
FIPV
PCR
RT-PCR
central nervous system (CNS)
diagnosis
discriminative
neurological signs
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
27 01 2021
27 01 2021
Historique:
received:
22
12
2020
revised:
15
01
2021
accepted:
21
01
2021
entrez:
30
1
2021
pubmed:
31
1
2021
medline:
12
2
2021
Statut:
epublish
Résumé
Cats with neurologic feline infectious peritonitis (FIP) are difficult to diagnose. Aim of this study was to evaluate the diagnostic value of detecting feline coronavirus (FCoV) RNA and spike (S) gene mutations in cerebrospinal fluid (CSF). The study included 30 cats with confirmed FIP (six with neurological signs) and 29 control cats (eleven with neurological signs) with other diseases resulting in similar clinical signs. CSF was tested for FCoV RNA by 7b-RT-qPCR in all cats. In RT-qPCR-positive cases, S-RT-qPCR was additionally performed to identify spike gene mutations. Nine cats with FIP (9/30, 30%), but none of the control cats were positive for FCoV RNA in CSF. Sensitivity of 7b-RT-qPCR in CSF was higher for cats with neurological FIP (83.3%; 95% confidence interval (95% CI) 41.8-98.9) than for cats with non-neurological FIP (16.7%; 95% CI 6.1-36.5). Spike gene mutations were rarely detected. FCoV RNA was frequently present in CSF of cats with neurological FIP, but only rarely in cats with non-neurological FIP. Screening for spike gene mutations did not enhance specificity in this patient group. Larger populations of cats with neurological FIP should be explored in future studies.
Sections du résumé
BACKGROUND
Cats with neurologic feline infectious peritonitis (FIP) are difficult to diagnose. Aim of this study was to evaluate the diagnostic value of detecting feline coronavirus (FCoV) RNA and spike (S) gene mutations in cerebrospinal fluid (CSF).
METHODS
The study included 30 cats with confirmed FIP (six with neurological signs) and 29 control cats (eleven with neurological signs) with other diseases resulting in similar clinical signs. CSF was tested for FCoV RNA by 7b-RT-qPCR in all cats. In RT-qPCR-positive cases, S-RT-qPCR was additionally performed to identify spike gene mutations.
RESULTS
Nine cats with FIP (9/30, 30%), but none of the control cats were positive for FCoV RNA in CSF. Sensitivity of 7b-RT-qPCR in CSF was higher for cats with neurological FIP (83.3%; 95% confidence interval (95% CI) 41.8-98.9) than for cats with non-neurological FIP (16.7%; 95% CI 6.1-36.5). Spike gene mutations were rarely detected.
CONCLUSIONS
FCoV RNA was frequently present in CSF of cats with neurological FIP, but only rarely in cats with non-neurological FIP. Screening for spike gene mutations did not enhance specificity in this patient group. Larger populations of cats with neurological FIP should be explored in future studies.
Identifiants
pubmed: 33513683
pii: v13020186
doi: 10.3390/v13020186
pmc: PMC7912268
pii:
doi:
Substances chimiques
RNA, Viral
0
Spike Glycoprotein, Coronavirus
0
spike protein, feline infectious peritonitis virus
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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