Simultaneous Determination of Antibodies to Pertussis Toxin and Adenylate Cyclase Toxin Improves Serological Diagnosis of Pertussis.

Pertussis in vitro diagnostics lateral flow multiplex point-of-care serology

Journal

Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402

Informations de publication

Date de publication:
27 Jan 2021
Historique:
received: 21 12 2020
revised: 22 01 2021
accepted: 22 01 2021
entrez: 30 1 2021
pubmed: 31 1 2021
medline: 31 1 2021
Statut: epublish

Résumé

Serological diagnosis of pertussis is mainly based on anti-pertussis toxin (PT) IgG antibodies. Since PT is included in all acellular vaccines (ACV), serological assays do not differentiate antibodies induced by ACVs and infection. Adenylate cyclase toxin (ACT) is not included in the ACVs, which makes it a promising candidate for pertussis serology with the specific aim of separating infection- and ACV-induced antibodies. A multiplex lateral flow test with PT and ACT antigens was developed to measure serum antibodies from pertussis-seropositive patients (

Identifiants

pubmed: 33513780
pii: diagnostics11020180
doi: 10.3390/diagnostics11020180
pmc: PMC7912298
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Aapo Knuutila (A)

Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland.

Alex-Mikael Barkoff (AM)

Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland.

Jussi Mertsola (J)

Department of Pediatrics and Adolescent Medicine, Turku University Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland.

Radim Osicka (R)

Laboratory of Molecular Biology of Bacterial Pathogens, Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.

Peter Sebo (P)

Laboratory of Molecular Biology of Bacterial Pathogens, Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic.

Qiushui He (Q)

Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland.
Department of Medical Microbiology, Capital Medical University, No. 10 Xi Tou Tiao, You'an Men Wai, Feng Tai District, Beijing 100069, China.
Research Center for Infections and Immunity, Institute of Biomedicine, University of Turku, 20520 Turku, Finland.

Classifications MeSH