Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris.
Candida auris
Emergence
qPCR
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
17
12
2020
accepted:
24
01
2021
pubmed:
31
1
2021
medline:
9
10
2021
entrez:
30
1
2021
Statut:
ppublish
Résumé
Candida auris is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of C. auris, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13 C. auris CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9 Candida spp. (70 strains). This system is suitable to correctly identify C. auris infections and to trace its source.
Identifiants
pubmed: 33515096
doi: 10.1007/s10096-021-04176-8
pii: 10.1007/s10096-021-04176-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1547-1551Références
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