Differentiation of seminiferous tubule-associated stem cells into leydig cell and myoid cell lineages.
Animals
Cell Differentiation
/ drug effects
Cell Lineage
/ drug effects
Cell Proliferation
/ drug effects
Leydig Cells
/ cytology
Male
Platelet-Derived Growth Factor
/ pharmacology
Rats, Sprague-Dawley
Receptors, Platelet-Derived Growth Factor
/ metabolism
Seminiferous Tubules
/ cytology
Thy-1 Antigens
/ metabolism
Leydig cells
Myoid cells
PDGF
Peritubular stem cells
Testis
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
05 04 2021
05 04 2021
Historique:
received:
31
03
2020
revised:
05
01
2021
accepted:
19
01
2021
pubmed:
31
1
2021
medline:
7
10
2021
entrez:
30
1
2021
Statut:
ppublish
Résumé
Peritubular stem Leydig cells (SLCs) have been identified from rat testicular seminiferous tubules. However, no stem cells for peritubular myoid cells have been reported in the adult testis so far. In the present study, we tested the hypothesis that the peritubular SLCs are multipotent and able to form either Leydig or myoid cells. Using cultured tubules, we show that in the presence of PDGFAA and luteinizing hormone, SLCs became testosterone-producing Leydig cells, while in the presence of PDGFBB and TGFB, the cells formed α-smooth muscle actin-expressing myoid cells. This multipotency was also confirmed by culture of isolated CD90
Identifiants
pubmed: 33515640
pii: S0303-7207(21)00023-X
doi: 10.1016/j.mce.2021.111179
pii:
doi:
Substances chimiques
Platelet-Derived Growth Factor
0
Thy-1 Antigens
0
Receptors, Platelet-Derived Growth Factor
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111179Informations de copyright
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