The porcine deltacoronavirus accessory protein NS6 is expressed in vivo and incorporated into virions.
Coronavirus
NS6
Porcine deltacoronavirus (PDCoV)
Virions
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
11
10
2020
revised:
17
01
2021
accepted:
18
01
2021
pubmed:
31
1
2021
medline:
13
3
2021
entrez:
30
1
2021
Statut:
ppublish
Résumé
Porcine deltacoronavirus (PDCoV) is one of the emerged coronaviruses posing a significant threat to the swine industry. Previous work showed the presence of a viral accessory protein NS6 in PDCoV-infected cells. In this study, we detected the expression of the NS6 protein in small intestinal tissues of PDCoV-infected piglets. In addition, SDS-PAGE and Western blot analysis of sucrose gradient-purified virions showed the presence of a 13-kDa NS6 protein. Further evidences of the presence of NS6 in the PDCoV virions were obtained by immunogold staining of purified virions with anti-NS6 antiserum, and by immunoprecipitation of NS6 from purified virions. Finally, the anti-NS6 antibody was not able to neutralize PDCoV in cultured cells. These data establish for the first time that the accessory protein NS6 is expressed during infection in vivo and incorporated into PDCoV virions.
Identifiants
pubmed: 33515858
pii: S0042-6822(21)00019-2
doi: 10.1016/j.virol.2021.01.011
pmc: PMC7825830
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Viral Nonstructural Proteins
0
nonstructural protein, coronavirus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-8Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
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