First pregnancies in jennies with vitrified donkey semen using a new warming method.
Fertility
Jack
Sperm
Thawing
Vitrification
Journal
Animal : an international journal of animal bioscience
ISSN: 1751-732X
Titre abrégé: Animal
Pays: England
ID NLM: 101303270
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
10
06
2020
revised:
30
09
2020
accepted:
01
10
2020
pubmed:
31
1
2021
medline:
25
3
2021
entrez:
30
1
2021
Statut:
ppublish
Résumé
Sperm vitrification has been recently developed, but fertility trials have not been performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response and fertility were compared to conventional freezing. In Experiment 1, sperm was vitrified in straws and warmed in 3 ml of extender or in a water bath at: 37 °C/30 s; 43 °C/10 s; and 60 °C/5 s. Sperm motility, plasma and acrosome membranes and DNA integrity were compared between treatments. In Experiment 2, jennies were inseminated twice (500 × 10
Identifiants
pubmed: 33516021
pii: S1751-7311(20)30099-9
doi: 10.1016/j.animal.2020.100097
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100097Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.