Salivary
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor
/ genetics
Carcinoma, Squamous Cell
/ diagnosis
Case-Control Studies
Early Detection of Cancer
Female
Gene Expression Regulation, Neoplastic
Humans
Leukoplakia, Oral
/ genetics
Lichen Planus, Oral
/ genetics
Male
Membrane Proteins
/ genetics
Middle Aged
Mouth Neoplasms
/ diagnosis
Neoplasm Staging
Saliva
/ chemistry
Sensitivity and Specificity
Up-Regulation
Biomarker
ciliogenesis and planar polarity effector 1 (CPLANE1)
early detection of cancer
oral squamous cell carcinoma
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
07
12
2020
revised:
12
01
2021
accepted:
13
01
2021
entrez:
31
1
2021
pubmed:
1
2
2021
medline:
7
2
2021
Statut:
ppublish
Résumé
This study aimed to identify novel biomarkers for oral squamous cell carcinoma (OSCC) screening to improve the survival rate of patients with oral cancer. We investigated differential salivary gene expression in patients with OSCC, those with oral potentially malignant disorders (OPMDs), and healthy volunteers (HVs). CPLANE1 was selected for further investigation by microarray analysis. We used quantitative reverse transcription PCR (qRT-PCR) to determine CPLANE1 expression levels in the saliva. The expression of CPLANE1 in normal and oral cancer tissues was analyzed using the Gene Expression database of Normal and Tumor tissues. qRT-PCR analysis of saliva samples showed that CPLANE1 expression levels were significantly higher in OSCC patients than in HVs and OPMDs patients. Furthermore, we developed a screening test for OSCC using CPLANE1 and showed that it had good accuracy. Salivary CPLANE1 could be a useful biomarker for OSCC screening and early detection.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
This study aimed to identify novel biomarkers for oral squamous cell carcinoma (OSCC) screening to improve the survival rate of patients with oral cancer.
MATERIALS AND METHODS
METHODS
We investigated differential salivary gene expression in patients with OSCC, those with oral potentially malignant disorders (OPMDs), and healthy volunteers (HVs). CPLANE1 was selected for further investigation by microarray analysis. We used quantitative reverse transcription PCR (qRT-PCR) to determine CPLANE1 expression levels in the saliva. The expression of CPLANE1 in normal and oral cancer tissues was analyzed using the Gene Expression database of Normal and Tumor tissues.
RESULTS
RESULTS
qRT-PCR analysis of saliva samples showed that CPLANE1 expression levels were significantly higher in OSCC patients than in HVs and OPMDs patients. Furthermore, we developed a screening test for OSCC using CPLANE1 and showed that it had good accuracy.
CONCLUSION
CONCLUSIONS
Salivary CPLANE1 could be a useful biomarker for OSCC screening and early detection.
Identifiants
pubmed: 33517281
pii: 41/2/765
doi: 10.21873/anticanres.14828
doi:
Substances chimiques
Biomarkers, Tumor
0
CPLANE1 protein, human
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
765-772Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.