Metabolic Glycoengineering Enables the Ultrastructural Visualization of Sialic Acids in the Glycocalyx of the Alveolar Epithelial Cell Line hAELVi.

alveolar epithelium bioorthogonal labeling electron microscopy glycocalyx hAELVi huAELVi metabolic glycoengineering sialic acid

Journal

Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513

Informations de publication

Date de publication:
2020
Historique:
received: 05 10 2020
accepted: 23 11 2020
entrez: 1 2 2021
pubmed: 2 2 2021
medline: 2 2 2021
Statut: epublish

Résumé

The glycocalyx-a plethora of sugars forming a dense layer that covers the cell membrane-is commonly found on the epithelial surface of lumen forming tissue. New glycocalyx specific properties have been defined for various organs in the last decade. However, in the lung alveolar epithelium, its structure and functions remain almost completely unexplored. This is partly due to the lack of physiologically relevant, cost effective

Identifiants

pubmed: 33520965
doi: 10.3389/fbioe.2020.614357
pmc: PMC7841390
doi:

Types de publication

Journal Article

Langues

eng

Pagination

614357

Informations de copyright

Copyright © 2021 Brandt, Timm, Gorenflos López, Kwame Abledu, Kuebler, Hackenberger, Ochs and Lopez-Rodriguez.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Raphael Brandt (R)

Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Sara Timm (S)

Core Facility Electron Microscopy, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Jacob L Gorenflos López (JL)

Department Chemical Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Department of Chemistry, Humboldt Universität zu Berlin, Berlin, Germany.

Jubilant Kwame Abledu (J)

Institute of Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Wolfgang M Kuebler (WM)

Institute of Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
German Center for Lung Research (DZL), Berlin, Germany.

Christian P R Hackenberger (CPR)

Department Chemical Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Department of Chemistry, Humboldt Universität zu Berlin, Berlin, Germany.

Matthias Ochs (M)

Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Core Facility Electron Microscopy, Charité - Universitätsmedizin Berlin, Berlin, Germany.
German Center for Lung Research (DZL), Berlin, Germany.

Elena Lopez-Rodriguez (E)

Institute of Functional Anatomy, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Classifications MeSH