Influence of Lactobacillus crispatus, Lactobacillus iners and Gardnerella vaginalis on bacterial vaginal composition in pregnant women.
Adult
Bacteria
Brazil
/ epidemiology
Female
Gardnerella vaginalis
/ isolation & purification
Humans
Lactobacillus
/ isolation & purification
Lactobacillus crispatus
/ isolation & purification
Polymerase Chain Reaction
/ methods
Pregnancy
Pregnancy Trimester, Second
Pregnant Women
Prevalence
Prospective Studies
RNA, Ribosomal, 16S
/ genetics
Vagina
/ microbiology
Vaginosis, Bacterial
/ epidemiology
Gardnerella vaginalis
Lactobacillus crispatus
Lactobacillus iners
Vaginal microbiome
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
16
07
2020
accepted:
19
01
2021
pubmed:
2
2
2021
medline:
29
10
2021
entrez:
1
2
2021
Statut:
ppublish
Résumé
To investigate associations between bacterial species in the vagina in mid-trimester pregnant women from Brazil. The vaginal microbiome in 613 subjects was identified by analysis of the V1-V3 region of bacterial 16S ribosomal RNA and the relative prevalence of individual bacteria were determined. The bacterial species present in the greatest number of women were Lactobacillus crispatus (306 women), L. iners (298 women) and Gardnerella vaginalis (179 women). When present in the vagina, L. crispatus was the most abundant bacterium more than 85% of the time. In contrast, L. iners and G. vaginalis were most abundant in 63% and 41% of women who were positive for these microorganisms, respectively (p < 0.0001 vs. L. crispatus). The proportion of L. crispatus was negatively associated with the proportions of L. iners, L. jensenii, L. gasseri, G. vaginalis, Megasphaera, Atopobium vaginae and Prevotella (p < 0.0001). In contrast, the proportion of G. vaginalis was positively associated with levels of Megasphaera, A. vaginae and Prevotella (p < 0.0001) while L. iners proportion was unrelated to the proportion of L. jensenii, G. vaginalis, Megasphaera, A. vaginae or Prevotella. The composition of the vaginal microbiota in mid-trimester pregnant women is influenced by the relative concentrations of L. crispatus, L. iners and G. vaginalis.
Identifiants
pubmed: 33521838
doi: 10.1007/s00404-021-05978-z
pii: 10.1007/s00404-021-05978-z
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
395-400Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM103324
Pays : United States
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
Références
DiGiulio DB, Callahan BJ, McMurdie PJ, Costello EK, Lyell DJ, Robaczewska A et al (2015) Temporal and spatial variation of the human microbiota during pregnancy. Proc Natl Acad Sci USA 112:11060–11065
doi: 10.1073/pnas.1502875112
Romero R, Hassan SS, Gajer P, Tarca AL, Fadrosh DW, Nikita L et al (2014) The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome 2(1):4
doi: 10.1186/2049-2618-2-4
MacIntyre DA, Chandiramani M, Lee YS, Kindinger L, Smith A, Angelopoulos N et al (2015) The vaginal microbiome during pregnancy and the postpartum period in a European population. Sci Rep 11(5):8988
doi: 10.1038/srep08988
Freitas AC, Chaban B, Bocking A, Rocco M, Yang S, Hill JE et al (2017) The vaginal microbiome of pregnant women is less rich and diverse, with lower prevalence of Molliccutes, compared to non-pregnant women. Sci Rep 7(1):9212
doi: 10.1038/s41598-017-07790-9
Witkin SS, Moron AF, Ridenhour BJ, Minis E, Hatanaka A, Sarmmento SGP et al (2019) Vaginal biomarkers that predict cervical length and dominant bacteria in the vaginal microbiome of pregnant women. mBio 10:e0224219
doi: 10.1128/mBio.02242-19
Romero R, Hassan SS, Gajer P, Tarca AL, Fadrosh DW, Bieda J et al (2014) The vaginal microbiota of pregnant women who subsequently have spontaneous preterm labor and delivery and those with a normal delivery at term. Microbiome 2:18
doi: 10.1186/2049-2618-2-18
Callahan BJ, DiGiulio DB, Goltsman DSA, Sun CL, Costello EK, Jeganathan P et al (2017) Replication and refinement of a vaginal microbial signature of preterm birth in two racially distinct cohorts of US women. Proc Natl Acad Sci USA 114(37):9966–9971
doi: 10.1073/pnas.1705899114
Stout MJ, Zhou Y, Wylie KM, Tarr PI, Macones GA, Tuuli MG (2017) Early pregnancy vaginal microbiome trends and preterm birth. Am J Obstet Gynecol 217(3):356.e1-356.e18
doi: 10.1016/j.ajog.2017.05.030
Faust K, Sathirapongsasuti JF, Izard J, Segata N, Gevers D, Raes J et al (2012) Microbial co-occurrence relationships in the human microbiome. PLoS Comput Biol 8(7):e1002606
doi: 10.1371/journal.pcbi.1002606
Fredricks DN, Fiedler TL, Marrazzo JM (2005) Molecular identification of bacteria associated with bacterial vaginosis. N Eng J Med 353:1899–1911
doi: 10.1056/NEJMoa043802
Srinivasan S, Hoffman NG, Morga MT, Matsen FA, Fiedler TL, Hall RW et al (2012) Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria. PLoS ONE 7:e37818
doi: 10.1371/journal.pone.0037818
KIndinger LM, MacIntyre DA, Lee YS, Machesi JR, Smith A, McDonald J et al (2016) Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage. Sci Transl Med 8(350):350
doi: 10.1126/scitranslmed.aag1026
Kindinger LM, Bennett PR, Lee YS, Machesi JR, Smith A, Cacciatore S et al (2017) The interaction between vaginal microbiota, cervical length, and vaginal progesterone treatment for preterm birth risk. Microbiome 5(1):6
doi: 10.1186/s40168-016-0223-9
Vitalli B, Cruciani F, Picone G, Parolin C, Donders G, Laghi L (2015) Vaginal microbiome and metabolome highlight specific signatures of bacterial vaginosis. Eur J Clin Microbiol Infect Dis 34:2367–2376
doi: 10.1007/s10096-015-2490-y
Srinivasan S, Morgan MT, Fiedler TL, Djukovic D, Hoffman NG, Raftery D et al (2015) Metabolic signatures of bacterial vaginosis. mBio 6:e00204-e215
doi: 10.1128/mBio.00204-15
Watson E, Reid G (2018) Metabolomics as a clinical testing method for the diagnosis of vaginal dysbiosis. Am J Reprod Immunol 80:e12979
doi: 10.1111/aji.12979
Cessarani C, Foschi C, Parolin C, D’Antuono A, Gaspari V, Consolandi C et al (2019) Diversity of vaginal microbiome and metabolome during genital infections. Sci Rep 9:14095
doi: 10.1038/s41598-019-50410-x
France MT, Mendes-Soares H, Forney LJ (2016) Genomic comparisons of Lactobacillus crispatus and lactobacillus iners reveal potential ecological drivers of community composition in the vagina. Appl Environ Microbiol 82:7063–7073
doi: 10.1128/AEM.02385-16
Witkin SS, Linhares IM (2017) Why do lactobacilli dominate the human vaginal microbiota? BJOG 124:606–611
doi: 10.1111/1471-0528.14390