The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.
CCAAT-enhancer-binding protein (C/EBP)
Zfat
adipocyte
lipid metabolism
transcription regulation
transgenic mice
Journal
Journal of cellular biochemistry
ISSN: 1097-4644
Titre abrégé: J Cell Biochem
Pays: United States
ID NLM: 8205768
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
22
12
2020
received:
09
06
2020
accepted:
23
12
2020
pubmed:
2
2
2021
medline:
5
10
2021
entrez:
1
2
2021
Statut:
ppublish
Résumé
Adipocytes play crucial roles in the control of whole-body energy homeostasis. Differentiation and functions of the adipocytes are regulated by various transcription factors. Zfat (zinc-finger protein with AT-hook) is a transcriptional regulator that controls messenger RNA expression of specific genes through binding to their transcription start sites. Here we report important roles of Zfat in the adipocytes. We establish inducible Zfat-knockout (Zfat iKO) mice where treatment with tamoxifen causes a marked reduction in Zfat expression in various tissues. Tamoxifen treatment of Zfat iKO mice reduces the white adipose tissues (WATs) mass, accompanied by the decreased triglyceride levels. Zfat is expressed in both the adipose-derived stem cells (ADSCs) and mature adipocytes in the WATs. In ex vivo assays of the mature adipocytes differentiated from the Zfat iKO ADSCs, loss of Zfat in the mature adipocytes reduces the triglyceride levels, suggesting cell autonomous roles of Zfat in the maintenance of the mature adipocytes. Furthermore, we identify the Atg13, Brf1, Psmc3, and Timm22 genes as Zfat-target genes in the mature adipocytes. In contrast, loss of Zfat in the ADSCs impairs adipocyte differentiation with the decreased expression of C/EBPα and adiponectin. Thus, we propose that Zfat plays crucial roles in maintenance and differentiation of the adipocytes.
Identifiants
pubmed: 33522619
doi: 10.1002/jcb.29890
pmc: PMC8248092
doi:
Substances chimiques
Adiponectin
0
CCAAT-Enhancer-Binding Proteins
0
Transcription Factors
0
ZFAT protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
626-638Informations de copyright
© 2021 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals LLC.
Références
Nucleic Acids Res. 2020 Nov 4;48(19):10848-10866
pubmed: 32997115
Diabetes. 2005 Jun;54(6):1744-54
pubmed: 15919796
Diabetes. 2004 Nov;53(11):2757-66
pubmed: 15504955
Trends Endocrinol Metab. 2012 Sep;23(9):435-43
pubmed: 22784416
Nature. 2002 Apr 18;416(6882):763-7
pubmed: 11961560
Semin Cell Dev Biol. 2018 Apr;76:142-153
pubmed: 28765093
Hum Mol Genet. 2004 Oct 1;13(19):2221-31
pubmed: 15294872
Biochim Biophys Acta. 2013 Feb;1833(2):274-85
pubmed: 22683763
Mol Biol Cell. 2009 Apr;20(7):1981-91
pubmed: 19211835
J Cell Biochem. 2015 Jan;116(1):149-57
pubmed: 25169027
PLoS One. 2010 Apr 08;5(4):e9983
pubmed: 20386695
J Struct Funct Genomics. 2015 Jun;16(2):55-65
pubmed: 25801860
J Biol Chem. 2016 Jul 15;291(29):15282-91
pubmed: 27226588
PLoS One. 2013 Oct 03;8(10):e76254
pubmed: 24098453
Cell Death Dis. 2017 Dec 14;8(12):3218
pubmed: 29242562
Trends Endocrinol Metab. 2012 Feb;23(2):56-64
pubmed: 22079269
Exp Physiol. 2014 Sep;99(9):1168-78
pubmed: 25128326
Anticancer Res. 2015 Aug;35(8):4419-23
pubmed: 26168481
Autophagy. 2014 Jun;10(6):944-56
pubmed: 24879146
Hum Genet. 2011 Dec;130(6):725-33
pubmed: 21626137
J Mol Biol. 2001 Jun 29;310(1):1-26
pubmed: 11419933
BMC Med Genomics. 2018 Jun 20;11(1):54
pubmed: 29925367
Am J Pathol. 2009 Sep;175(3):927-39
pubmed: 19700756
Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14199-204
pubmed: 20660741
Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19860-5
pubmed: 19910529
Biochem Biophys Res Commun. 2012 Aug 17;425(1):107-12
pubmed: 22828507
J Cell Biochem. 2021 Jun;122(6):626-638
pubmed: 33522619
Autophagy. 2009 Nov;5(8):1118-30
pubmed: 19844159
Genomics. 2008 May;91(5):451-7
pubmed: 18329245
J Biol Regul Homeost Agents. 2013 Jul-Sep;27(3):729-38
pubmed: 24152840
Mol Biol Cell. 2009 Apr;20(7):1992-2003
pubmed: 19225151
Int J Mol Med. 2018 Nov;42(5):2595-2603
pubmed: 30106088
Biochim Biophys Acta. 2016 Nov;1859(11):1398-1410
pubmed: 27591365
J Clin Immunol. 2012 Dec;32(6):1243-52
pubmed: 22706687