The Rab32/BLOC-3-dependent pathway mediates host defense against different pathogens in human macrophages.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
05
02
2020
accepted:
24
11
2020
entrez:
1
2
2021
pubmed:
2
2
2021
medline:
20
4
2022
Statut:
epublish
Résumé
Macrophages provide a first line of defense against microorganisms, and while some mechanisms to kill pathogens such as the oxidative burst are well described, others are still undefined or unknown. Here, we report that the Rab32 guanosine triphosphatase and its guanine nucleotide exchange factor BLOC-3 (biogenesis of lysosome-related organelles complex-3) are central components of a trafficking pathway that controls both bacterial and fungal intracellular pathogens. This host-defense mechanism is active in both human and murine macrophages and is independent of well-known antimicrobial mechanisms such as the NADPH (reduced form of nicotinamide adenine dinucleotide phosphate)-dependent oxidative burst, production of nitric oxide, and antimicrobial peptides. To survive in human macrophages,
Identifiants
pubmed: 33523895
pii: 7/3/eabb1795
doi: 10.1126/sciadv.abb1795
pmc: PMC7810368
pii:
doi:
Substances chimiques
Guanine Nucleotide Exchange Factors
0
Rab32 protein, human
EC 3.6.1.-
Rab32 protein, mouse
EC 3.6.1.-
rab GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
Références
Cell Host Microbe. 2014 Aug 13;16(2):249-256
pubmed: 25121752
Cell Host Microbe. 2016 Feb 10;19(2):216-26
pubmed: 26867180
Adv Exp Med Biol. 2016;915:283-94
pubmed: 27193549
Annu Rev Microbiol. 2014;68:415-38
pubmed: 25002086
Microbiology (Reading). 2010 Dec;156(Pt 12):3689-3698
pubmed: 20817644
Infect Immun. 1991 Sep;59(9):2901-8
pubmed: 1879916
Infect Immun. 1996 Jul;64(7):2765-73
pubmed: 8698506
J Innate Immun. 2019;11(3):216-226
pubmed: 30943492
Science. 2012 Nov 16;338(6109):960-3
pubmed: 23162001
N Engl J Med. 2009 Dec 31;361(27):2609-18
pubmed: 20018961
Immunology. 2014 Jan;141(1):96-110
pubmed: 24088176
PLoS Pathog. 2019 Jun 14;15(6):e1007879
pubmed: 31199852
Sci Rep. 2015 Jul 30;5:12524
pubmed: 26224331
Annu Rev Microbiol. 2014;68:317-36
pubmed: 25208300
Biochim Biophys Acta. 2016 Mar;1858(3):546-66
pubmed: 26556394
Small GTPases. 2018 May 4;9(3):216-223
pubmed: 27645564
Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18418-23
pubmed: 22042847
J Infect. 2014 Jan;68 Suppl 1:S38-50
pubmed: 24119827
Science. 2020 Jul 24;369(6502):450-455
pubmed: 32703879
PLoS One. 2015 May 06;10(5):e0124307
pubmed: 25946027
Science. 2013 Feb 15;339(6121):823-6
pubmed: 23287722
Cell Host Microbe. 2017 Aug 9;22(2):217-231
pubmed: 28799907
Cell Mol Immunol. 2015 Jan;12(1):5-23
pubmed: 25263488
Front Cell Infect Microbiol. 2017 Aug 09;7:348
pubmed: 28848711
Nat Genet. 2015 Mar;47(3):267-71
pubmed: 25642632
Mol Microbiol. 1994 Aug;13(4):555-68
pubmed: 7997169
Methods. 2011 Apr;53(4):331-8
pubmed: 21288739
J Bacteriol. 2002 Oct;184(19):5457-67
pubmed: 12218034
Annu Rev Biochem. 2008;77:755-76
pubmed: 18173371
Bioinformatics. 2015 Sep 15;31(18):3078-80
pubmed: 25979474