Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
01 02 2021
01 02 2021
Historique:
entrez:
2
2
2021
pubmed:
3
2
2021
medline:
3
11
2021
Statut:
epublish
Résumé
In this procedure, synthesis of cDNA is performed in the presence of saturating concentrations of all four dNTPs and trace amounts of a single radiolabeled dNTP. After subtraction hybridization, the enriched single-stranded cDNA is radiolabeled to high specific activity in a second synthetic reaction by extension of random oligonucleotide primers using the Klenow fragment of
Identifiants
pubmed: 33526416
pii: 2021/2/pdb.prot100644
doi: 10.1101/pdb.prot100644
doi:
Substances chimiques
DNA Probes
0
DNA, Complementary
0
Oligonucleotides
0
Radioisotopes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021 Cold Spring Harbor Laboratory Press.