Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable Region.


Journal

Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414

Informations de publication

Date de publication:
26 01 2021
Historique:
received: 21 12 2020
revised: 21 01 2021
accepted: 24 01 2021
entrez: 3 2 2021
pubmed: 4 2 2021
medline: 23 7 2021
Statut: epublish

Résumé

In vivo clinical applications of nanobodies (VHHs) require molecules that induce minimal immunoresponse and therefore possess sequences as similar as possible to the human VH domain. Although the relative sequence variability in llama nanobodies has been used to identify scaffolds with partially humanized signature, the transformation of the

Identifiants

pubmed: 33530572
pii: biom11020163
doi: 10.3390/biom11020163
pmc: PMC7911018
pii:
doi:

Substances chimiques

Single-Domain Antibodies 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : National Key Research and Development Program of China
ID : 2019YFA0905600
Organisme : Javna Agencija za Raziskovalno dejavnost Republike Slovenije
ID : ARRS/N4-0046
Organisme : Javna Agencija za Raziskovalno dejavnost Republike Slovenije
ID : ARRS/J4-9322
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 18510

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Auteurs

Miguel A Soler (MA)

CONCEPT Lab, Italian Institute of Technology (IIT), 16152 Genova, Italy.

Barbara Medagli (B)

Department of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy.

Jiewen Wang (J)

Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China.

Sandra Oloketuyi (S)

Lab of Environmental and Life Sciences, University of Nova Gorica, 5000 Rožna Dolina-Nova Gorica, Slovenia.

Gregor Bajc (G)

Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.

He Huang (H)

Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China.

Sara Fortuna (S)

Department of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy.

Ario de Marco (A)

Lab of Environmental and Life Sciences, University of Nova Gorica, 5000 Rožna Dolina-Nova Gorica, Slovenia.

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Classifications MeSH