Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Biofilms
/ drug effects
Drug Resistance, Bacterial
Gene Expression Regulation, Bacterial
/ drug effects
Humans
Molecular Docking Simulation
Molecular Dynamics Simulation
Quorum Sensing
/ drug effects
Salicylic Acid
/ pharmacology
Staphylococcal Infections
/ drug therapy
Staphylococcus aureus
/ drug effects
Trans-Activators
/ genetics
Virulence Factors
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 02 2021
03 02 2021
Historique:
received:
01
07
2020
accepted:
19
01
2021
entrez:
4
2
2021
pubmed:
5
2
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Salicylic acid (SAL) has recently been shown to induce biofilm formation in Staphylococcus aureus and to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatory agr system and its impact on S. aureus biofilm formation. The agr quorum-sensing system, which is a central regulator in S. aureus pathogenicity, plays a pivotal role in the dispersal of S. aureus mature biofilms and contributes to the creation of new colonization sites. Here, we demonstrate that SAL impairs biofilm dispersal by interfering with agr expression. As revealed by our work, protease and surfactant molecule production is diminished, and bacterial cell autolysis is also negatively affected by SAL. Furthermore, as a consequence of SAL treatment, the S. aureus biofilm matrix revealed the lack of extracellular DNA. In silico docking and simulation of molecular dynamics provided evidence for a potential interaction of AgrA and SAL, resulting in reduced activity of the agr system. In conclusion, SAL stabilized the mature S. aureus biofilms, which may prevent bacterial cell dissemination. However, it may foster the establishment of infections locally and consequently increase bacterial persistence leading to therapeutic failure.
Identifiants
pubmed: 33536503
doi: 10.1038/s41598-021-82308-y
pii: 10.1038/s41598-021-82308-y
pmc: PMC7858585
doi:
Substances chimiques
Agr protein, Staphylococcus aureus
0
Anti-Bacterial Agents
0
Bacterial Proteins
0
Trans-Activators
0
Virulence Factors
0
Salicylic Acid
O414PZ4LPZ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2953Références
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