Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 02 2021
03 02 2021
Historique:
received:
24
06
2020
accepted:
18
01
2021
entrez:
4
2
2021
pubmed:
5
2
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Despite our expanding knowledge about the mechanism underlying atrial fibrillation (AF), the interplay between the biological events underlying AF remains incompletely understood. This study aimed to identify the functionally enriched gene-sets in AF and capture their interconnection via pivotal factors, that may drive or be driven by AF. Global abundance of the proteins in the left atrium of AF patients compared to control patients (n = 3/group), and the functionally enriched biological processes in AF were determined by mass-spectrometry and gene set enrichment analysis, respectively. The data were validated in an independent cohort (n = 19-20/group). In AF, the gene-sets of innate immune system, metabolic process, cellular component disassembly and ion homeostasis were up-regulated, while the gene-set of ciliogenesis was down-regulated. The innate immune system was over-represented by neutrophil degranulation, the components of which were extensively shared by other gene-sets altered in AF. In the independent cohort, an activated form of neutrophils was more present in the left atrium of AF patients with the increased gene expression of neutrophil granules. MYH10, required for ciliogenesis, was decreased in the atrial fibroblasts of AF patients. We report the increased neutrophil degranulation appears to play a pivotal role, and affects multiple biological processes altered in AF.
Identifiants
pubmed: 33536523
doi: 10.1038/s41598-021-82533-5
pii: 10.1038/s41598-021-82533-5
pmc: PMC7859227
doi:
Substances chimiques
Nonmuscle Myosin Type IIB
EC 3.6.1.-
nonmuscle myosin type IIB heavy chain
EC 3.6.1.-
Myosin Heavy Chains
EC 3.6.4.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2972Subventions
Organisme : ZonMw
ID : 016.146.310
Pays : Netherlands
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