DNA methylation patterns identify subgroups of pancreatic neuroendocrine tumors with clinical association.


Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
03 02 2021
Historique:
received: 29 04 2020
accepted: 02 11 2020
entrez: 4 2 2021
pubmed: 5 2 2021
medline: 10 8 2021
Statut: epublish

Résumé

Here we report the DNA methylation profile of 84 sporadic pancreatic neuroendocrine tumors (PanNETs) with associated clinical and genomic information. We identified three subgroups of PanNETs, termed T1, T2 and T3, with distinct patterns of methylation. The T1 subgroup was enriched for functional tumors and ATRX, DAXX and MEN1 wild-type genotypes. The T2 subgroup contained tumors with mutations in ATRX, DAXX and MEN1 and recurrent patterns of chromosomal losses in half of the genome with no association between regions with recurrent loss and methylation levels. T2 tumors were larger and had lower methylation in the MGMT gene body, which showed positive correlation with gene expression. The T3 subgroup harboured mutations in MEN1 with recurrent loss of chromosome 11, was enriched for grade G1 tumors and showed histological parameters associated with better prognosis. Our results suggest a role for methylation in both driving tumorigenesis and potentially stratifying prognosis in PanNETs.

Identifiants

pubmed: 33536587
doi: 10.1038/s42003-020-01469-0
pii: 10.1038/s42003-020-01469-0
pmc: PMC7859232
doi:

Substances chimiques

Biomarkers, Tumor 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

155

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NHGRI NIH HHS
ID : U54 HG003273
Pays : United States
Organisme : Cancer Research UK
ID : C29717/A17263
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C29717/A18484
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C596/A18076
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C596/A20921
Pays : United Kingdom

Investigateurs

Maria Scardoni (M)
Claudio Bassi (C)
Sonia Grimaldi (S)
Cinzia Cantù (C)
Giada Bonizzato (G)
Samantha Bersani (S)
Davide Antonello (D)
Liliana Piredda (L)
Nicola Sperandio (N)
Stefano Barbi (S)
Paola Merlini (P)

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Auteurs

Vanessa Lakis (V)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Rita T Lawlor (RT)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy.

Felicity Newell (F)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Ann-Marie Patch (AM)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Andrea Mafficini (A)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy.

Anguraj Sadanandam (A)

Division of Molecular Pathology, The Institute of Cancer Research, London, UK.
The Royal Marsden Hospital, London, UK.

Lambros T Koufariotis (LT)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Rebecca L Johnston (RL)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Conrad Leonard (C)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Scott Wood (S)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Borislav Rusev (B)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy.

Vincenzo Corbo (V)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy.
Section of Pathology, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.

Claudio Luchini (C)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy.
Section of Pathology, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.

Sara Cingarlini (S)

ENETS Center of Excellence, University and Hospital Trust of Verona, Verona, Italy.
Section of Oncology, Department of Medicine, University and Hospital Trust of Verona, Verona, Italy.

Luca Landoni (L)

ENETS Center of Excellence, University and Hospital Trust of Verona, Verona, Italy.
The Pancreas Institute, University and Hospital Trust of Verona, Verona, Italy.

Roberto Salvia (R)

ENETS Center of Excellence, University and Hospital Trust of Verona, Verona, Italy.
The Pancreas Institute, University and Hospital Trust of Verona, Verona, Italy.

Michele Milella (M)

ENETS Center of Excellence, University and Hospital Trust of Verona, Verona, Italy.
Section of Oncology, Department of Medicine, University and Hospital Trust of Verona, Verona, Italy.

David Chang (D)

Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Academic Unit of Surgery, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow Royal Infirmary, Glasgow, G4 OSF, UK.
West of Scotland Pancreatic Unit, Glasgow Royal Infirmary, Glasgow, G31 2ER, UK.

Peter Bailey (P)

Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Department of General Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120, Heidelberg, Baden-Württemberg, Germany.

Nigel B Jamieson (NB)

Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Academic Unit of Surgery, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow Royal Infirmary, Glasgow, G4 OSF, UK.
West of Scotland Pancreatic Unit, Glasgow Royal Infirmary, Glasgow, G31 2ER, UK.

Fraser Duthie (F)

Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Department of Pathology, Queen Elizabeth University Hospital, Greater Glasgow & Clyde NHS, Glasgow, G51 4TF, UK.

Marie-Claude Gingras (MC)

Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, MS226, Houston, Texas, 77030-3411, USA.
Michael E. DeBakey Department of Surgery and The Elkins Pancreas Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030-3411, USA.

Donna M Muzny (DM)

Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, MS226, Houston, Texas, 77030-3411, USA.

David A Wheeler (DA)

Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, MS226, Houston, Texas, 77030-3411, USA.

Richard A Gibbs (RA)

Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, MS226, Houston, Texas, 77030-3411, USA.
Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, 77030, USA.

Massimo Milione (M)

Department of Pathology, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milan, Italy.

Paolo Pederzoli (P)

Ospedale Pederzoli, Peschiera del Garda, Verona, Italy.

Jaswinder S Samra (JS)

University of Sydney, Sydney, New South Wales, 2006, Australia.

Anthony J Gill (AJ)

University of Sydney, Sydney, New South Wales, 2006, Australia.
The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 370 Victoria Street, Darlinghurst, Sydney, New South Wales, 2010, Australia.

Amber L Johns (AL)

The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 370 Victoria Street, Darlinghurst, Sydney, New South Wales, 2010, Australia.

John V Pearson (JV)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.

Andrew V Biankin (AV)

Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.

Sean M Grimmond (SM)

University of Melbourne Centre for Cancer Research, Victorian Comprehensive Cancer Centre, 305 Grattan Street, Melbourne, Victoria, 3000, Australia.

Nicola Waddell (N)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia.
Institute for Molecular Bioscience, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia.

Katia Nones (K)

QIMR Berghofer Medical Research Institute, 300 Herston Road, Brisbane, Queensland, 4006, Australia. Katia.Nones@qimrberghofer.edu.au.

Aldo Scarpa (A)

ARC-Net Centre for Applied Research on Cancer, University and Hospital Trust of Verona, Verona, 37134, Italy. aldo.scarpa@univr.it.
Section of Pathology, Department of Diagnostics and Public Health, University of Verona, Verona, Italy. aldo.scarpa@univr.it.
ENETS Center of Excellence, University and Hospital Trust of Verona, Verona, Italy. aldo.scarpa@univr.it.

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