Breast milk urea as a nitrogen source for urease positive Bifidobacterium infantis.
Bifidobacterium
human milk
infant gut microbiota
urea
urease
Journal
FEMS microbiology ecology
ISSN: 1574-6941
Titre abrégé: FEMS Microbiol Ecol
Pays: England
ID NLM: 8901229
Informations de publication
Date de publication:
08 03 2021
08 03 2021
Historique:
received:
16
09
2020
accepted:
03
02
2021
pubmed:
5
2
2021
medline:
21
4
2021
entrez:
4
2
2021
Statut:
ppublish
Résumé
Human milk stimulates a health-promoting gut microbiome in infants. However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk. Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome. Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN). We found that B. longum subsp. infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium. We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp. infantis through proteomics. To the same end, metagenome data were mined for urease-related genes. It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase). Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants. These experiments show how an important gut commensal that colonizes the infant intestine can metabolize urea. The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.
Identifiants
pubmed: 33538807
pii: 6128667
doi: 10.1093/femsec/fiab019
pmc: PMC7947585
pii:
doi:
Substances chimiques
Oligosaccharides
0
Urea
8W8T17847W
Urease
EC 3.5.1.5
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.
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