Novel three-dimensional biochip pulmonary sarcoidosis model.

Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface.

Déclaration de conflit d'intérêts

The authors have read the journal’s policy and the authors of this manuscript have the following competing interests: BE is a paid employee of Genix-Engineering. MM was awarded a research grant from Mallinckrodt Pharmaceuticals and is an advisory board member of Mallinckrodt Pharmaceuticals. MM has a pending patent for this technology (Provisional patent application UMIP-441 Mirsaeidi 126683-040PV1). This does not alter our adherence to PLOS ONE policies on sharing data and materials.