Single Strain High-Depth NGS Reveals High rDNA (ITS-LSU) Variability in the Four Prevalent Pathogenic Species of the Genus
Candida albicans
ITS
LSU
Maldi-Tof
NCAC
Sanger
intragenomic rRNA variation
next generation sequencing (NGS)
typing
yeast
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
02 Feb 2021
02 Feb 2021
Historique:
received:
31
12
2020
revised:
27
01
2021
accepted:
29
01
2021
entrez:
5
2
2021
pubmed:
6
2
2021
medline:
6
2
2021
Statut:
epublish
Résumé
Ribosomal RNA in fungi is encoded by a series of genes and spacers included in a large operon present in 100 tandem repeats, normally in a single locus. The multigene nature of this locus was somehow masked by Sanger sequencing, which produces a single sequence reporting the prevalent nucleotide of each site. The introduction of next generation sequencing led to deeper knowledge of the individual sequences (reads) and therefore of the variants between the same DNA sequences located in different tandem repeats. In this framework, NGS sequencing of the rDNA region was used to elucidate the extent of intra- and inter-genomic variation at both the strain and species level. Specifically, the use of an innovative NGS technique allowed the high-throughput high-depth sequencing of the ITS1-LSU D1/D2 amplicons of 252 strains belonging to four opportunistic yeast species of the genus
Identifiants
pubmed: 33540602
pii: microorganisms9020302
doi: 10.3390/microorganisms9020302
pmc: PMC7912828
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Gilead Sciences
ID : Caratterizzazione del biofilm ed efficacia del trattamento con Amfotericina B liposomiale in ceppi filmogeni del gruppo Candida parapsilosis sensu lato (URL: http://www.itfellowshipprogram.it)
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