Distinct clonal lineages and within-host diversification shape invasive Staphylococcus epidermidis populations.
Aged
Aged, 80 and over
Bacterial Proteins
/ genetics
Biofilms
/ growth & development
Cross Infection
/ genetics
Female
Genotype
Hemolysis
Humans
Interspersed Repetitive Sequences
Male
Middle Aged
Mutation
Nasal Mucosa
/ metabolism
Phenotype
Staphylococcal Infections
/ genetics
Staphylococcus epidermidis
/ classification
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
11
09
2020
accepted:
11
01
2021
revised:
18
02
2021
pubmed:
6
2
2021
medline:
24
6
2021
entrez:
5
2
2021
Statut:
epublish
Résumé
S. epidermidis is a substantial component of the human skin microbiota, but also one of the major causes of nosocomial infection in the context of implanted medical devices. We here aimed to advance the understanding of S. epidermidis genotypes and phenotypes conducive to infection establishment. Furthermore, we investigate the adaptation of individual clonal lines to the infection lifestyle based on the detailed analysis of individual S. epidermidis populations of 23 patients suffering from prosthetic joint infection. Analysis of invasive and colonizing S. epidermidis provided evidence that invasive S. epidermidis are characterized by infection-supporting phenotypes (e.g. increased biofilm formation, growth in nutrient poor media and antibiotic resistance), as well as specific genetic traits. The discriminating gene loci were almost exclusively assigned to the mobilome. Here, in addition to IS256 and SCCmec, chromosomally integrated phages was identified for the first time. These phenotypic and genotypic features were more likely present in isolates belonging to sequence type (ST) 2. By comparing seven patient-matched nasal and invasive S. epidermidis isolates belonging to identical genetic lineages, infection-associated phenotypic and genotypic changes were documented. Besides increased biofilm production, the invasive isolates were characterized by better growth in nutrient-poor media and reduced hemolysis. By examining several colonies grown in parallel from each infection, evidence for genetic within-host population heterogeneity was obtained. Importantly, subpopulations carrying IS insertions in agrC, mutations in the acetate kinase (AckA) and deletions in the SCCmec element emerged in several infections. In summary, these results shed light on the multifactorial processes of infection adaptation and demonstrate how S. epidermidis is able to flexibly repurpose and edit factors important for colonization to facilitate survival in hostile infection environments.
Identifiants
pubmed: 33544760
doi: 10.1371/journal.ppat.1009304
pii: PPATHOGENS-D-20-02020
pmc: PMC7891712
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009304Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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