Pigment structure in the light-harvesting protein of the siphonous green alga Codium fragile.
Codium fragile
Resonance Raman
SCP
Siphonaxanthin
Siphonein
Siphonous green algae
Journal
Biochimica et biophysica acta. Bioenergetics
ISSN: 1879-2650
Titre abrégé: Biochim Biophys Acta Bioenerg
Pays: Netherlands
ID NLM: 101731706
Informations de publication
Date de publication:
01 05 2021
01 05 2021
Historique:
received:
13
10
2020
revised:
11
01
2021
accepted:
21
01
2021
pubmed:
6
2
2021
medline:
28
9
2021
entrez:
5
2
2021
Statut:
ppublish
Résumé
The siphonaxanthin-siphonein-chlorophyll-a/b-binding protein (SCP), a trimeric light-harvesting complex isolated from photosystem II of the siphonous green alga Codium fragile, binds the carotenoid siphonaxanthin (Sx) and/or its ester siphonein in place of lutein, in addition to chlorophylls a/b and neoxanthin. SCP exhibits a higher content of chlorophyll b (Chl-b) than its counterpart in green plants, light-harvesting complex II (LHCII), increasing the relative absorption of blue-green light for photosynthesis. Using low temperature absorption and resonance Raman spectroscopies, we reveal the presence of two non-equivalent Sx molecules in SCP, and assign their absorption peaks at 501 and 535 nm. The red-absorbing Sx population exhibits a significant distortion that is reminiscent of lutein 2 in trimeric LHCII. Unexpected enhancement of the Raman modes of Chls-b in SCP allows an unequivocal description of seven to nine non-equivalent Chls-b, and six distinct Chl-a populations in this protein.
Identifiants
pubmed: 33545114
pii: S0005-2728(21)00017-7
doi: 10.1016/j.bbabio.2021.148384
pii:
doi:
Substances chimiques
Light-Harvesting Protein Complexes
0
Photosystem II Protein Complex
0
Pigments, Biological
0
Xanthophylls
0
Chlorophyll
1406-65-1
siphonaxanthin
28526-44-5
chlorophyll b
5712ZB110R
Chlorophyll A
YF5Q9EJC8Y
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
148384Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.