Growth factor-eluting hydrogels for management of corneal defects.
Cornea
Defect
Growth factor
Hydrogel
Wound healing
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
24
05
2020
revised:
27
08
2020
accepted:
03
12
2020
entrez:
6
2
2021
pubmed:
7
2
2021
medline:
13
5
2021
Statut:
ppublish
Résumé
With 1.5-2.0 million new cases annually worldwide, corneal injury represents a common cause of vision loss, often from irreversible scarring due to surface corneal defects. In this study, we assessed the use of hepatocyte growth factor (HGF) loaded into an in situ photopolymerizable transparent gelatin-based hydrogel for the management of corneal defects. In vitro release kinetics showed that, in regard to the total amount of HGF released over a month, 55 ± 11% was released during the first 24 h, followed by a slow release profile for up to one month. The effect of HGF was assessed using an ex vivo model of pig corneal defect. After three days of organ culture, epithelial defects were found to be completely healed for 89% of the corneas treated with HGF, compared to only 11% of the corneas that had fully re-epithelialized when treated with the hydrogel without HGF. The thickness of the epithelial layer was found to be significantly higher for the HGF-treated group compared to the group treated with hydrogel without HGF (p = 0.0012). Finally, histological and immunostaining assessments demonstrated a better stratification and adhesion of the epithelial layer in the presence of HGF. These results suggest that the HGF-loaded hydrogel system represents a promising solution for the treatment of persistent corneal defects at risk of scarring.
Identifiants
pubmed: 33545916
pii: S0928-4931(20)33709-7
doi: 10.1016/j.msec.2020.111790
pmc: PMC7867677
mid: NIHMS1656185
pii:
doi:
Substances chimiques
Hydrogels
0
Hepatocyte Growth Factor
67256-21-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111790Subventions
Organisme : NIBIB NIH HHS
ID : R01 EB023052
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL140618
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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