Severe acute respiratory syndrome coronavirus 2 prevalence in saliva and gastric and intestinal fluid in patients undergoing gastrointestinal endoscopy in coronavirus disease 2019 endemic areas: Prospective cross-sectional study in Japan.


Journal

Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society
ISSN: 1443-1661
Titre abrégé: Dig Endosc
Pays: Australia
ID NLM: 9101419

Informations de publication

Date de publication:
Jan 2022
Historique:
revised: 01 02 2021
received: 25 12 2020
accepted: 02 02 2021
pubmed: 7 2 2021
medline: 14 1 2022
entrez: 6 2 2021
Statut: ppublish

Résumé

Gastrointestinal endoscopy (GIE) is useful for the early detection and treatment of many diseases; however, GIE is considered a high-risk procedure in the coronavirus disease 2019 (COVID-19) pandemic era. This study aimed to explore the rate of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positivity in saliva and gastrointestinal fluids to which endoscopy medical staff are exposed. The study was a single-center cross-sectional study. From June 1 to July 31, 2020, all patients who underwent GIE at Yokohama City University Hospital were registered. All patients provided 3 mL of saliva. For upper GIE, 10 mL of gastric fluid was collected through the endoscope. For lower GIE, 10 mL of intestinal fluid was collected through the endoscope. The primary outcome was the positive rate of SARS-CoV-2 in saliva and gastrointestinal fluids. We also analyzed serum-specific antibodies for SARS-CoV-2 and patients' background information. A total of 783 samples (560 upper GIE and 223 lower GIE samples) were analyzed. Polymerase chain reaction (PCR) on saliva samples did not show any positive results in either upper or lower GIE samples. However, 2.0% (16/783) of gastrointestinal fluid samples tested positive for SARS-CoV-2. No significant differences in age, sex, purpose of endoscopy, medication, or rate of antibody test positivity were found between PCR positive and PCR negative cases. Asymptomatic patients, even those with no detectable virus in their saliva, had SARS-CoV-2 in their gastrointestinal tract. Endoscopy medical staff should be aware of infection when performing procedures. The study was registered as UMIN000040587.

Identifiants

pubmed: 33548095
doi: 10.1111/den.13945
pmc: PMC8014498
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

96-104

Subventions

Organisme : Daiwa Securities Health Foundation
Organisme : The Japanese Foundation for Research and Promotion of Endoscopy (JFE)

Informations de copyright

© 2021 The Authors. Digestive Endoscopy published by John Wiley & Sons Australia, Ltd on behalf of Japan Gastroenterological Endoscopy Society.

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Auteurs

Shigeta Miyake (S)

Departments of, Neurosurgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Keiichi Ashikari (K)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Shingo Kato (S)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.
Clinical Cancer Genomics, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Tomohiro Takatsu (T)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Hirofumi Kuwashima (H)

Gastroenterology, Yokohama City University, Kanagawa, Japan.

Hiroaki Kaneko (H)

Gastroenterology, Yokohama City University, Kanagawa, Japan.

Koki Nagai (K)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Ikue Watari (I)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Takamitsu Sato (T)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Yutaro Yamaoka (Y)

Department of Microbiology, School of Medicine, Yokohama City University, Kanagawa, Japan.
Life Science Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Kanagawa, Japan.

Tetsuya Yamamoto (T)

Departments of, Neurosurgery, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Akihide Ryo (A)

Department of Microbiology, School of Medicine, Yokohama City University, Kanagawa, Japan.

Shin Maeda (S)

Gastroenterology, Yokohama City University, Kanagawa, Japan.

Atsushi Nakajima (A)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

Takuma Higurashi (T)

Gastroenterology and Hepatology, Graduate School of Medicine, Yokohama City University, Kanagawa, Japan.

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