Kinetics and catalytic efficiency of soil fluorescein diacetate hydrolase under the pesticide parathion stress.
Enzyme kinetics
Inhibition constants
Parathion
Soil FDA hydrolase
Soil properties
Journal
The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500
Informations de publication
Date de publication:
01 Jun 2021
01 Jun 2021
Historique:
received:
12
09
2020
revised:
18
12
2020
accepted:
19
12
2020
pubmed:
7
2
2021
medline:
19
3
2021
entrez:
6
2
2021
Statut:
ppublish
Résumé
Fluorescein diacetate hydrolase (FDA-H) is an accurate biochemical method measuring the total microbial activity in soil, which indicates soil quality under ambient environmental changes such as pesticide parathion (PTH). However, the influence of PTH on the kinetics of FDA-H is still unknown. In this study, fifteen farmland soils were exposed to acute PTH pollution to investigate how the kinetic characteristics of FDA-H change with PTH concentration. Results showed that PTH strongly inhibited the FDA-H activities. The values of maximum reaction velocity (V
Identifiants
pubmed: 33548707
pii: S0048-9697(20)38368-6
doi: 10.1016/j.scitotenv.2020.144835
pii:
doi:
Substances chimiques
Fluoresceins
0
Pesticides
0
Soil
0
Soil Pollutants
0
Parathion
61G466064D
Hydrolases
EC 3.-
diacetylfluorescein
YL39R93PRE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144835Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.