Mechanisms of DEET gustation in Drosophila.

Animals DEET / pharmacology Drosophila Proteins / genetics Drosophila melanogaster / drug effects Feeding Behavior / drug effects Insect Repellents / pharmacology Larva / drug effects Mutation Oviposition / drug effects Receptors, Cell Surface / genetics Taste / drug effects
Bitter gustatory receptors DEET Gr89a Gustatory receptors

Journal

Insect biochemistry and molecular biology
ISSN: 1879-0240
Titre abrégé: Insect Biochem Mol Biol
Pays: England
ID NLM: 9207282

Informations de publication

Date de publication:
04 2021
Historique:
received: 16 10 2020
revised: 31 01 2021
accepted: 31 01 2021
pubmed: 8 2 2021
medline: 30 7 2021
entrez: 7 2 2021
Statut: ppublish

Résumé

DEET is the most widely used active ingredient in insect repellents and offers protection against insect bites. We previously reported that DEET suppresses the feeding behavior of Drosophila, which is guided by gustatory receptors (GRs) in bitter-sensing gustatory receptor neurons. Here, we sought to identify new candidates using egg-laying assays. Upon screening all GR mutants, GR89a was identified as a potential DEET receptor. Gr89a mutants exhibited reduced oviposition avoidance, feeding avoidance, and electrophysiological responses compared to Gr32a, Gr33a, and Gr66a mutants. However, GR89a was found to modulate DEET avoidance, as demonstrated by genetic and RNA interference assays. Furthermore, we found that DEET ingestion severely affected larval and pupal development and survival, and therefore may act as an effective larvicide.

Identifiants

DOI: 10.1016/j.ibmb.2021.103550 PMID: 33549816
pubmed: 33549816
pii: S0965-1748(21)00033-3
doi: 10.1016/j.ibmb.2021.103550
pii:
doi:

Substances chimiques

Drosophila Proteins 0
Insect Repellents 0
Receptors, Cell Surface 0
DEET 134-62-3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103550

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Bhanu Shrestha (B)

Department of Bio & Fermentation Convergence Technology, Kookmin University, Seoul, 02707, Republic of Korea.

Youngseok Lee (Y)

Department of Bio & Fermentation Convergence Technology, Kookmin University, Seoul, 02707, Republic of Korea; Interdisciplinary Program for Bio-Health Convergence, Kookmin University, Seoul, 02707, Republic of Korea. Electronic address: ylee@kookmin.ac.kr.

Articles similaires

Meal Timing and Anthropometric and Metabolic Outcomes: A Systematic Review and Meta-Analysis.

Hiu Yee Liu, Ashley A Eso, Nathan Cook et al.
1.00
Humans Meals Time Factors Female Adult

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Benzoates › Benzamides › N,N-Diéthyl-méta-toluamide › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Phénomènes physiologiques › Alimentation et nutrition › Phénomènes physiologiques nutritionnels › Comportement alimentaire › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Pesticides › Insectifuges › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Oviposition › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Mechanisms of DEET gustation in Drosophila.
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Sensation › Goût › Mechanisms of DEET gustation in Drosophila.