Identification of madangamine A as a novel lysosomotropic agent to inhibit autophagy.


Journal

Bioorganic & medicinal chemistry
ISSN: 1464-3391
Titre abrégé: Bioorg Med Chem
Pays: England
ID NLM: 9413298

Informations de publication

Date de publication:
15 03 2021
Historique:
received: 05 12 2020
revised: 18 01 2021
accepted: 20 01 2021
pubmed: 8 2 2021
medline: 21 9 2021
entrez: 7 2 2021
Statut: ppublish

Résumé

Madangamines are marine natural products isolated from Xestospongia ingens, and madangamine A-E with a different D-ring structure have been reported. We have reported that madangamine A has strong anti-proliferative activity against various human cancer cell lines. In this study, to clarify the anti-proliferative activity of madangamine A, we searched for molecular target of the madangamine A in human cells. Treatment with madangamine A increased the levels of LC3-II and p62, autophagy-related proteins, concomitant with growth inhibition. Moreover, madangamine A resulted in lysosome enlargement and increase in lysosomal pH, which are same phenomena observed in chloroquine-treated cells. These results suggest that madangamine A is a novel lysosome inhibitor, and the anti-proliferative activity of madangamine A is due to the inhibition of lysosome function.

Identifiants

pubmed: 33549907
pii: S0968-0896(21)00049-3
doi: 10.1016/j.bmc.2021.116041
pii:
doi:

Substances chimiques

Biological Products 0
Heterocyclic Compounds, 4 or More Rings 0
MAP1LC3B protein, human 0
Microtubule-Associated Proteins 0
madangamine A 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

116041

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Kazuki Miura (K)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan.

Sayaka Kawano (S)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan.

Takahiro Suto (T)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan.

Takaaki Sato (T)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan.

Noritaka Chida (N)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan.

Siro Simizu (S)

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 223-8522, Japan. Electronic address: simizu@applc.keio.ac.jp.

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Classifications MeSH