Structural basis of enzyme activity regulation by the propeptide of l-lysine α-oxidase precursor from

Crystal structure L-Lysine α-oxidase LAAO, l-amino acid oxidase LysOX, l-lysine α-oxidase Precursor Substrate recognition

Journal

Journal of structural biology: X
ISSN: 2590-1524
Titre abrégé: J Struct Biol X
Pays: United States
ID NLM: 101761384

Informations de publication

Date de publication:
2021
Historique:
received: 14 10 2020
revised: 17 12 2020
accepted: 07 01 2021
entrez: 8 2 2021
pubmed: 9 2 2021
medline: 9 2 2021
Statut: epublish

Résumé

Harmuful proteins are usually synthesized as inactive precursors and are activated by proteolytic processing. l-Amino acid oxidase (LAAO) is a flavoenzyme that catalyzes the oxidative deamination of l-amino acid to produce a 2-oxo acid with ammonia and highly toxic hydrogen peroxide and, therefore, is expressed as a precursor. The LAAO precursor shows significant variation in size and the cleavage pattern for activation. However, the molecular mechanism of how the propeptide suppresses the enzyme activity remains unclear except for deaminating/decarboxylating

Identifiants

pubmed: 33554108
doi: 10.1016/j.yjsbx.2021.100044
pii: S2590-1524(21)00001-5
pmc: PMC7844570
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100044

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Auteurs

Masaki Kitagawa (M)

Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.

Nanako Ito (N)

Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.

Yuya Matsumoto (Y)

Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.

Masaya Saito (M)

Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.

Takashi Tamura (T)

Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.

Hitoshi Kusakabe (H)

Enzyme Sensor Co., Ltd., Tsukuba, Ibaraki 305-0047, Japan.

Kenji Inagaki (K)

Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan.

Katsumi Imada (K)

Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.

Classifications MeSH