Dynamic effects of genetic variation on gene expression revealed following hypoxic stress in cardiomyocytes.
cardiomyocytes
chromosomes
eQTL
gene expression
gene regulation
genetic variation
genetics
genomics
human
hypoxia
stress response
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
08 02 2021
08 02 2021
Historique:
received:
28
03
2020
accepted:
06
02
2021
pubmed:
9
2
2021
medline:
9
2
2022
entrez:
8
2
2021
Statut:
epublish
Résumé
One life-threatening outcome of cardiovascular disease is myocardial infarction, where cardiomyocytes are deprived of oxygen. To study inter-individual differences in response to hypoxia, we established an in vitro model of induced pluripotent stem cell-derived cardiomyocytes from 15 individuals. We measured gene expression levels, chromatin accessibility, and methylation levels in four culturing conditions that correspond to normoxia, hypoxia, and short- or long-term re-oxygenation. We characterized thousands of gene regulatory changes as the cells transition between conditions. Using available genotypes, we identified 1,573 genes with a
Identifiants
pubmed: 33554857
doi: 10.7554/eLife.57345
pii: 57345
pmc: PMC7906610
doi:
pii:
Substances chimiques
Oxygen
S88TT14065
Banques de données
GEO
['GSE144426']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL092206
Pays : United States
Organisme : NIA NIH HHS
ID : F31 AG044948
Pays : United States
Informations de copyright
© 2021, Ward et al.
Déclaration de conflit d'intérêts
MW, NB, AS, MS, YG No competing interests declared
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