High-resolution melting PCR assay as a powerful tool for the epidemiological surveillance of tularemia in Western Europe.
Francisella tularensis
High-resolution melting PCR
Surveillance
Tularemia
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
31
07
2020
revised:
14
12
2020
accepted:
26
01
2021
pubmed:
9
2
2021
medline:
12
1
2022
entrez:
8
2
2021
Statut:
ppublish
Résumé
In Europe, tularemia is caused by Francisella tularensis subsp. holarctica and is a sporadic disease affecting mainly wildlife animals and humans. Classification of this species relies on canonical single nucleotide polymorphisms (canSNPs). Four main clades have been described for F. tularensis subsp. holarctica: B.4, B.6, B.12 and B.16. Phylogeographic studies have shown that clade B.6 is predominant in Western Europe and B.12 in Eastern and Central Europe. Based on this global phylogeny, we aimed to design a molecular typing assay for all genetic subclades of subclade B.11, which is the predominant subclade in clade B.6. We designed high-resolution melting (HRM) primers for the screening of 109 canSNPs divided in seven orders of discrimination for the molecular epidemiology analysis and tracking of Francisella tularensis subsp. holarctica in Western Europe.
Identifiants
pubmed: 33556556
pii: S1567-1348(21)00038-1
doi: 10.1016/j.meegid.2021.104741
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104741Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.