Role of MUC1 in lubrication of pleural mesothelial cells cultured on fibrine gel.
Dexamethasone
Fibrine gel
Friction
MUC1
Mesothelial cells
Neuraminidase
Pleura
Journal
Tissue & cell
ISSN: 1532-3072
Titre abrégé: Tissue Cell
Pays: Scotland
ID NLM: 0214745
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
07
10
2020
revised:
12
01
2021
accepted:
23
01
2021
pubmed:
9
2
2021
medline:
30
11
2021
entrez:
8
2
2021
Statut:
ppublish
Résumé
To elucidate the role of sialomucin in friction reduction, we investigated the sliding friction of pleural mesothelial cells monolayers cultured on fibrine gel. These measurements were performed on normal (4/4 RM-4) and on tumor (CARM-L1 TG3) cell lines. The effect of treatment with neuraminidase, which removes sialic acid from sialomucin, and of dexamethasone, which has shown to increase sialomucin expression, were also assessed. Furthermore, the expression of the main form of cell-surface-associated mucin (MUC1) present in the mesothelium, was assessed by western blot and immunofluorescence, under different experimental conditions. Expression of MUC1 was not significantly different in the two cell lines. Moreover, dexamethasone did not increase the expression of MUC1. Coefficient of kinetic friction (μ) was significantly higher in tumor cells than in normal cells. Neuraminidase increased μ in both cell lines. These results suggest that sialomucin may play a role in reducing the friction of pleural mesothelial cells.
Identifiants
pubmed: 33556842
pii: S0040-8166(21)00019-7
doi: 10.1016/j.tice.2021.101503
pii:
doi:
Substances chimiques
MUC1 protein, human
0
Mucin-1
0
Sialomucins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
101503Informations de copyright
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