Molecular characterization and serodiagnostic potential of Echinococcus granulosus hexokinase.
Animals
Echinococcosis
/ diagnosis
Echinococcus granulosus
/ enzymology
Enzyme-Linked Immunosorbent Assay
Escherichia coli
/ genetics
Female
Hexokinase
/ genetics
Humans
Immunoglobulin G
/ blood
Mice
Mice, Inbred BALB C
Sensitivity and Specificity
Serologic Tests
/ methods
Specific Pathogen-Free Organisms
Diagnosis
Echinococcus granulosus
Hexokinase
Immunogenicity
Immunolocalization
Indirect ELISA
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
08 Feb 2021
08 Feb 2021
Historique:
received:
30
09
2020
accepted:
23
01
2021
entrez:
9
2
2021
pubmed:
10
2
2021
medline:
20
8
2021
Statut:
epublish
Résumé
Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus (sensu stricto), is a life-threatening but neglected zoonosis. Glycolytic enzymes are crucial molecules for the survival and development of E. granulosus. The aim of this study was to investigate the molecular characterization, immunogenicity, tissue distribution and serodiagnostic potential of E. granulosus hexokinase (EgHK), the first key enzyme in the glycolytic pathway. EgHK was cloned and expressed in Escherichia coli. Specific serum antibodies were evaluated in mice immunized with recombinant EgHK (rEgHK). The location of EgHK in the larval stage of E. granulosus was determined using fluorescence immunohistochemistry, and the potential of rEgHK as a diagnostic antigen was investigated in patients with CE using indirect enzyme-linked immunosorbent assay (ELISA). Recombinant EgHK could be identified in the sera of patients with CE and in mouse anti-rEgHK sera. High titers of specific immunoglobulin G were induced in mice after immunization with rEgHK. EgHK was mainly located in the tegument, suckers and hooklets of protoscoleces and in the germinal layer and laminated layer of the cyst wall. The sensitivity and specificity of the rEgHK-ELISA reached 91.3% (42/46) and 87.8% (43/49), respectively. We have characterized the sequence, structure and location of EgHK and investigated the immunoreactivity, immunogenicity and serodiagnostic potential of rEgHK. Our results suggest that EgHK may be a promising candidate for the development of vaccines against E. granulosus and an effective antigen for the diagnosis of human CE.
Sections du résumé
BACKGROUND
BACKGROUND
Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus (sensu stricto), is a life-threatening but neglected zoonosis. Glycolytic enzymes are crucial molecules for the survival and development of E. granulosus. The aim of this study was to investigate the molecular characterization, immunogenicity, tissue distribution and serodiagnostic potential of E. granulosus hexokinase (EgHK), the first key enzyme in the glycolytic pathway.
METHODS
METHODS
EgHK was cloned and expressed in Escherichia coli. Specific serum antibodies were evaluated in mice immunized with recombinant EgHK (rEgHK). The location of EgHK in the larval stage of E. granulosus was determined using fluorescence immunohistochemistry, and the potential of rEgHK as a diagnostic antigen was investigated in patients with CE using indirect enzyme-linked immunosorbent assay (ELISA).
RESULTS
RESULTS
Recombinant EgHK could be identified in the sera of patients with CE and in mouse anti-rEgHK sera. High titers of specific immunoglobulin G were induced in mice after immunization with rEgHK. EgHK was mainly located in the tegument, suckers and hooklets of protoscoleces and in the germinal layer and laminated layer of the cyst wall. The sensitivity and specificity of the rEgHK-ELISA reached 91.3% (42/46) and 87.8% (43/49), respectively.
CONCLUSIONS
CONCLUSIONS
We have characterized the sequence, structure and location of EgHK and investigated the immunoreactivity, immunogenicity and serodiagnostic potential of rEgHK. Our results suggest that EgHK may be a promising candidate for the development of vaccines against E. granulosus and an effective antigen for the diagnosis of human CE.
Identifiants
pubmed: 33557934
doi: 10.1186/s13071-021-04606-8
pii: 10.1186/s13071-021-04606-8
pmc: PMC7869421
doi:
Substances chimiques
Immunoglobulin G
0
Hexokinase
EC 2.7.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105Subventions
Organisme : Fundamental Research Funds for the Central Universities
ID : lzujbky-2020-50
Organisme : Natural Science Foundation of Gansu Province (CN)
ID : 20JR10RA636
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