Daturataturin A, a withanolide in Datura metel L., induces HaCaT autophagy through the PI3K-Akt-mTOR signaling pathway.
autophagy
daturataturin A
inflammation
proliferation
senescence
Journal
Phytotherapy research : PTR
ISSN: 1099-1573
Titre abrégé: Phytother Res
Pays: England
ID NLM: 8904486
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
revised:
02
06
2020
received:
05
03
2020
accepted:
07
10
2020
pubmed:
10
2
2021
medline:
20
4
2021
entrez:
9
2
2021
Statut:
ppublish
Résumé
Daturataturin A (DTA), a withanolide compound in Datura metel L., exhibits excellent anti-inflammatory and anti-proliferative activities. Here, we report the study of DTA-induced proliferation and inflammation in human immortalized keratinocytes (HaCaTs) and the associated molecular mechanisms. HaCaTs are a model of the epidermal proliferative state of cells. The pharmacodynamics and mechanism of DTA were studied by western blot, immunofluorescence, apoptosis and proliferation detection, and real-time quantitative polymerase chain reaction. We confirmed that DTA induced HaCaT autophagy, which, in turn, induced HaCaT senescence and, ultimately, led to cell cycle arrest. DTA also negatively regulated inflammation through the activation of autophagy. This may be one of the mechanisms underlying the action of Datura metel L. preparation used for the treatment of psoriasis.
Substances chimiques
Withanolides
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1546-1558Subventions
Organisme : Major State Basic Research Development Program of China
ID : 2013CB531801
Organisme : National Key R&D Program of China
ID : 2018YFC1707105
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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