In vitro antiplatelet activity of extract and its fractions of Paulownia Clone in Vitro 112 leaves.
Blood Platelets
/ drug effects
Cloning, Molecular
Female
Glucosides
/ pharmacology
Humans
Lamiales
/ genetics
Lipid Peroxidation
/ drug effects
Male
Phenols
/ pharmacology
Plant Extracts
/ isolation & purification
Plant Leaves
Plants, Genetically Modified
/ genetics
Platelet Activation
/ drug effects
Platelet Adhesiveness
/ drug effects
Platelet Aggregation
/ drug effects
Platelet Aggregation Inhibitors
/ isolation & purification
Secondary Metabolism
Adhesion
Aggregation
Paulownia
Platelet activation
Platelets
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
20
11
2020
revised:
15
01
2021
accepted:
18
01
2021
pubmed:
10
2
2021
medline:
20
7
2021
entrez:
9
2
2021
Statut:
ppublish
Résumé
Paulownia Clone in Vitro 112, also known as Oxytree is a hybrid of Paulownia elongata and Paulownia fortunei, developed under laboratory conditions. Its seeds are sterile, making it a noninvasive variety that can only be propagated in the laboratory. In China, species from the Paulownia genus (Paulowniaceae) are widely used in traditional medicine for the treatment of infectious diseases, such as gonorrhea and erysipelas. It has a broad spectrum of bioactivity, including neuroprotective, antioxidant, antibacterial, antiphlogistic, antiviral, and cytotoxic actions. However, the antiplatelet potential of Paulownia Clone in Vitro 112 has not yet been described. The aim of our study was thus to examine the effect of an extract and four fractions from leaves of Paulownia Clone in Vitro 112 on various parameters of platelet activation in an in vitro model. Composition of the investigated extract and fractions was determined by UHPLC-UV-MS. The following parameters of platelet activation were investigated: nonenzymatic lipid peroxidation in resting platelets; enzymatic lipid peroxidation (AA metabolism) in platelets activated by thrombin; superoxide anion (O Verbascoside a phenylethnanoid glycoside, was the main secondary metabolite of the extract from leaves of oxytree (constituting approximately 45 % of all compounds). There were also iridoids, such as catalpol, aucubin, and 7-hydroxytomentoside, as well as flavonoids, such as luteolin and apigenin glycosides. Moreover, the extract had stronger antiplatelet properties than the fractions. For example, the extract at 10 μg/mL inhibited five parameters of platelet activation. Our results show that Paulownia Clone in Vitro 112 leaves are a new valuable source of compounds with antiplatelet potential.
Sections du résumé
BACKGROUND
BACKGROUND
Paulownia Clone in Vitro 112, also known as Oxytree is a hybrid of Paulownia elongata and Paulownia fortunei, developed under laboratory conditions. Its seeds are sterile, making it a noninvasive variety that can only be propagated in the laboratory. In China, species from the Paulownia genus (Paulowniaceae) are widely used in traditional medicine for the treatment of infectious diseases, such as gonorrhea and erysipelas. It has a broad spectrum of bioactivity, including neuroprotective, antioxidant, antibacterial, antiphlogistic, antiviral, and cytotoxic actions. However, the antiplatelet potential of Paulownia Clone in Vitro 112 has not yet been described.
STUDY DESIGN
METHODS
The aim of our study was thus to examine the effect of an extract and four fractions from leaves of Paulownia Clone in Vitro 112 on various parameters of platelet activation in an in vitro model.
METHODS
METHODS
Composition of the investigated extract and fractions was determined by UHPLC-UV-MS. The following parameters of platelet activation were investigated: nonenzymatic lipid peroxidation in resting platelets; enzymatic lipid peroxidation (AA metabolism) in platelets activated by thrombin; superoxide anion (O
RESULTS
RESULTS
Verbascoside a phenylethnanoid glycoside, was the main secondary metabolite of the extract from leaves of oxytree (constituting approximately 45 % of all compounds). There were also iridoids, such as catalpol, aucubin, and 7-hydroxytomentoside, as well as flavonoids, such as luteolin and apigenin glycosides. Moreover, the extract had stronger antiplatelet properties than the fractions. For example, the extract at 10 μg/mL inhibited five parameters of platelet activation.
CONCLUSIONS
CONCLUSIONS
Our results show that Paulownia Clone in Vitro 112 leaves are a new valuable source of compounds with antiplatelet potential.
Identifiants
pubmed: 33561640
pii: S0753-3322(21)00086-X
doi: 10.1016/j.biopha.2021.111301
pii:
doi:
Substances chimiques
Glucosides
0
Phenols
0
Plant Extracts
0
Platelet Aggregation Inhibitors
0
acteoside
3TGX09BD5B
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111301Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.