The Age-Sensitive Efficacy of Calorie Restriction on Mitochondrial Biogenesis and mtDNA Damage in Rat Liver.
age-sensitive efficacy of CR
aging
calorie restriction
mitochondrial biogenesis
mtDNA damage
rat liver
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Feb 2021
07 Feb 2021
Historique:
received:
28
12
2020
revised:
03
02
2021
accepted:
04
02
2021
entrez:
10
2
2021
pubmed:
11
2
2021
medline:
13
4
2021
Statut:
epublish
Résumé
Calorie restriction (CR) is the most efficacious treatment to delay the onset of age-related changes such as mitochondrial dysfunction. However, the sensitivity of mitochondrial markers to CR and the age-related boundaries of CR efficacy are not fully elucidated. We used liver samples from ad libitum-fed (AL) rats divided in: 18-month-old (AL-18), 28-month-old (AL-28), and 32-month-old (AL-32) groups, and from CR-treated (CR) 28-month-old (CR-28) and 32-month-old (CR-32) counterparts to assay the effect of CR on several mitochondrial markers. The age-related decreases in citrate synthase activity, in TFAM, MFN2, and DRP1 protein amounts and in the mtDNA content in the AL-28 group were prevented in CR-28 counterparts. Accordingly, CR reduced oxidative mtDNA damage assessed through the incidence of oxidized purines at specific mtDNA regions in CR-28 animals. These findings support the anti-aging effect of CR up to 28 months. Conversely, the protein amounts of LonP1, Cyt c, OGG1, and APE1 and the 4.8 Kb mtDNA deletion content were not affected in CR-28 rats. The absence of significant differences between the AL-32 values and the CR-32 counterparts suggests an age-related boundary of CR efficacy at this age. However, this only partially curtails the CR benefits in counteracting the generalized aging decline and the related mitochondrial involvement.
Identifiants
pubmed: 33562258
pii: ijms22041665
doi: 10.3390/ijms22041665
pmc: PMC7915472
pii:
doi:
Substances chimiques
DNA, Mitochondrial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : FFABR2018
Références
Biogerontology. 2014;15(5):417-38
pubmed: 25015781
PLoS One. 2013 Sep 13;8(9):e74644
pubmed: 24058615
Nutrition. 1989 May-Jun;5(3):155-71; discussion 172
pubmed: 2520283
Oncotarget. 2015 Jul 20;6(20):17923-37
pubmed: 26053100
Int J Mol Sci. 2019 Apr 15;20(8):
pubmed: 30991726
Aging Cell. 2015 Aug;14(4):497-510
pubmed: 25902704
J Biol Chem. 2011 Sep 16;286(37):31975-83
pubmed: 21768646
Redox Biol. 2013 Feb 09;1:258-64
pubmed: 24024159
Int J Mol Sci. 2019 Sep 03;20(17):
pubmed: 31484398
Trends Endocrinol Metab. 2016 Feb;27(2):105-117
pubmed: 26754340
Mech Ageing Dev. 2009 May;130(5):343-9
pubmed: 19428453
Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18410-5
pubmed: 20930118
Cells. 2020 Jul 16;9(7):
pubmed: 32708786
Oxid Med Cell Longev. 2013;2013:528935
pubmed: 23577224
Mol Cell. 2013 Jan 10;49(1):121-32
pubmed: 23201127
Cell. 2013 Jun 6;153(6):1194-217
pubmed: 23746838
Aging Cell. 2020 Feb;19(2):e13080
pubmed: 31833194
Circ Res. 2018 Sep 14;123(7):905-924
pubmed: 30355076
J Gerontol A Biol Sci Med Sci. 2019 May 16;74(6):760-769
pubmed: 30010806
J Physiol. 2016 Apr 15;594(8):2043-60
pubmed: 26607973
Mech Ageing Dev. 2018 Sep;174:47-54
pubmed: 29427568
Mutat Res. 1992 Sep;275(3-6):181-93
pubmed: 1383760
J Gerontol A Biol Sci Med Sci. 2015 Apr;70(4):399-409
pubmed: 24691092
Free Radic Biol Med. 2016 Jul;96:78-88
pubmed: 27091693
Free Radic Biol Med. 2018 Aug 20;124:447-453
pubmed: 29969715
Exp Gerontol. 2016 Dec 1;85:33-40
pubmed: 27620821
Free Radic Biol Med. 2012 Dec 1;53(11):2043-53
pubmed: 23000245
Eur J Biochem. 2003 May;270(10):2295-302
pubmed: 12752449
Gene. 1998 Mar 16;209(1-2):23-30
pubmed: 9524209
Biochim Biophys Acta. 2014 Jul;1840(7):2184-91
pubmed: 24631828
Ageing Res Rev. 2017 Oct;39:15-28
pubmed: 28610949
Mitochondrion. 2015 Nov;25:67-75
pubmed: 26437364
Nucleic Acids Res. 1999 Apr 15;27(8):1935-42
pubmed: 10101204
Antioxid Redox Signal. 2013 Jul 20;19(3):310-20
pubmed: 22901095
Aging Cell. 2017 Aug;16(4):624-633
pubmed: 28544158
Int J Mol Sci. 2019 Jul 05;20(13):
pubmed: 31284385
Free Radic Biol Med. 2001 Apr 15;30(8):916-23
pubmed: 11295534
Age (Dordr). 2013 Oct;35(5):1607-20
pubmed: 22945739
Mech Ageing Dev. 2010 May;131(5):330-7
pubmed: 20363243
J Biol Chem. 2000 Feb 4;275(5):3343-7
pubmed: 10652323
Mol Cell. 2013 Jan 10;49(1):186-99
pubmed: 23201123
Clin Interv Aging. 2017 Nov 08;12:1887-1902
pubmed: 29184395
Trends Cell Biol. 2015 Mar;25(3):158-70
pubmed: 25499735
Free Radic Biol Med. 2016 Nov;100:188-198
pubmed: 27387767
J Gerontol A Biol Sci Med Sci. 2005 Jun;60(6):715-23
pubmed: 15983173
J Cell Sci. 2010 Mar 15;123(Pt 6):917-26
pubmed: 20179104
J Gerontol A Biol Sci Med Sci. 2017 Nov 9;72(12):1638-1646
pubmed: 28531280
Biochim Biophys Acta. 2015 Nov;1847(11):1434-47
pubmed: 25979234
PLoS Genet. 2016 Mar 15;12(3):e1005939
pubmed: 26978189
Ageing Res Rev. 2009 Jul;8(3):173-88
pubmed: 19491041
Ageing Res Rev. 2019 Sep;54:100940
pubmed: 31415807
Int J Mol Sci. 2019 May 27;20(10):
pubmed: 31137890
Cold Spring Harb Perspect Biol. 2015 Dec 01;7(12):
pubmed: 26626938