Multiscale three-dimensional imaging of intact human organs down to the cellular scale using hierarchical phase-contrast tomography.


Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
03 Feb 2021
Historique:
entrez: 10 2 2021
pubmed: 11 2 2021
medline: 11 2 2021
Statut: epublish

Résumé

Human organs are complex, three-dimensional and multiscale systems. Spatially mapping the human body down through its hierarchy, from entire organs to their individual functional units and specialised cells, is a major obstacle to fully understanding health and disease. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique utilising the European Synchrotron Radiation Facility's Extremely Brilliant Source: the world's first high-energy 4

Identifiants

pubmed: 33564772
doi: 10.1101/2021.02.03.429481
pmc: PMC7872374
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : F32 HL134229
Pays : United States
Organisme : Medical Research Council
ID : MR/S007687/1
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R01 HL094567
Pays : United States

Commentaires et corrections

Type : UpdateIn

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Auteurs

C Walsh (C)

Centre for Advanced Biomedical Imaging, University College London, U.K.

P Tafforeau (P)

European Synchrotron Radiation Facility, Grenoble, France.

Willi L Wagner (WL)

Dept of Diagnostic and Interventional Radiology, University Hospital Heidelberg, Heidelberg, Germany Translational Lung Research Centre Heidelberg (TLRC), German Lung Research Centre (DZL), Heidelberg, Germany.

D J Jafree (DJ)

Developmental Biology and Cancer Programme, Great Ormond Street Institute of Child Health, University College London, UK.
UCL MB/PhD Programme, Faculty of Medical Sciences, University College London, UK.

A Bellier (A)

French Alps Laboratory of Anatomy (LADAF), Grenoble Alpes University, Grenoble, France.

C Werlein (C)

Institute of Pathology, Hannover Medical School, Hannover, Germany (Carl-Neuberg-Straße 1, 30625 Hannover).

M P Kühnel (MP)

Institute of Pathology, Hannover Medical School, Hannover, Germany (Carl-Neuberg-Straße 1, 30625 Hannover).
Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH).

E Boller (E)

European Synchrotron Radiation Facility, Grenoble, France.

S Walker-Samuel (S)

Centre for Advanced Biomedical Imaging, University College London, U.K.

J L Robertus (JL)

Department of Histopathology, Royal Brompton and Harefield NHS Foundation Trust, London, UK.
National Heart & Lung Institute, Imperial College London, London, UK.

D A Long (DA)

Developmental Biology and Cancer Programme, Great Ormond Street Institute of Child Health, University College London, UK.

J Jacob (J)

Centre for Medical Image Computing, University College London, London, UK.
Department of Radiology, University College London Hospitals NHS Foundation Trust, London, UK.

S Marussi (S)

Department of Mechanical Engineering University College London, U.K.

E Brown (E)

Centre for Advanced Biomedical Imaging, University College London, U.K.

N Holroyd (N)

Centre for Advanced Biomedical Imaging, University College London, U.K.

D D Jonigk (DD)

Institute of Pathology, Hannover Medical School, Hannover, Germany (Carl-Neuberg-Straße 1, 30625 Hannover).
Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH).

M Ackermann (M)

Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, Mainz.

P D Lee (PD)

Department of Mechanical Engineering University College London, U.K.

Classifications MeSH