How human serum albumin-selective DNA aptamer binds to bovine and canine serum albumins.


Journal

Biopolymers
ISSN: 1097-0282
Titre abrégé: Biopolymers
Pays: United States
ID NLM: 0372525

Informations de publication

Date de publication:
Mar 2021
Historique:
revised: 12 01 2021
received: 15 09 2020
accepted: 14 01 2021
pubmed: 11 2 2021
medline: 26 10 2021
entrez: 10 2 2021
Statut: ppublish

Résumé

Serum albumin (SA) is the most abundant carrier protein in blood. SA carries a diverse range of nutrients, drugs, and metal ions. It has wide clinical and biochemical applications. Human serum albumin (HSA) can be used as a biomarker for kidney and liver diseases. Aptasensor is one of potential HSA detection methods. HSA-specific aptamer was selected for HSA detection. In animals, bovine serum albumin (BSA) and canine serum albumins (CSA) share high sequence similarities to HSA. Thus, it is interesting to explore the possibility of using HSA-selective aptamer for BSA and CSA aptasensor. In this study, molecular dynamics (MD) simulations were initially employed to investigate the binding of aptamer to BSA and CSA in comparison to HSA. Like HSA, both BSA and CSA can bind aptamer, but different binding affinities are observed. BSA shows the tighter binding to aptamer than CSA. Domain III is found to be the aptamer-binding domain although no specific aptamer conformation is captured. However, in all cases, the aptamer utilizes the 3'-end to attach on an albumin surface. Both nucleobases and phosphate backbones on a DNA aptamer are important for albumin-aptamer complexation. Our results imply the possibility of using HSA-specific aptamer for BSA detection due to tighter binding observed, but may be less effective in CSA. However, the test in actual complicated condition must be further studied.

Identifiants

pubmed: 33565613
doi: 10.1002/bip.23421
doi:

Substances chimiques

Aptamers, Nucleotide 0
Serum Albumin 0
Serum Albumin, Bovine 27432CM55Q

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e23421

Subventions

Organisme : Development of Advance Researcher Competence System for Competitiveness in Agriculture and Food from Kasetsart University Research and Development Institute (KURDI)
ID : FF(KU)25.64
Organisme : National Nanotechnology Center
ID : P1751330
Organisme : National Science and Technology Development Agency (NSTDA)
ID : SCA-CO-2562-9727-TH
Organisme : Office of National Higher Education Science Research and Innovation policy council and PMUB
ID : B05F630035

Informations de copyright

© 2021 Wiley Periodicals LLC.

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Auteurs

Nattapon Kuntip (N)

Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.

Deanpen Japrung (D)

National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Khlong Luang, Pathum Thani, Thailand.

Prapasiri Pongprayoon (P)

Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.

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