Methods to purify DNA from extracellular vesicles: Focus on exosomes.
Biomarker
Cargo
Cell-cell communication
DNA isolation
Exosomes
Extracellular vesicles
Nucleic acids
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
10
2
2021
pubmed:
11
2
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Exosomes are extracellular vesicles secreted by cells and involved in intercellular communications among close and distant cells. Exosomes encapsulate and carry biomolecules as cargo to the recipient cells. They contain nucleic acids (DNA, RNA, microRNA) proteins and lipids. Each exosomal components may be isolated and be studied by specific techniques. In this chapter, different methods will be described to isolate DNA from exosomes, since it is important in shaping the response of the recipient cells following the exosome uptake in multiple scenarios, including physiological and pathological conditions. Moreover, the exosomal DNA may be a novel biomarker for diagnosis, disease progression and patient's treatment response.
Identifiants
pubmed: 33565966
pii: S0076-6879(20)30326-8
doi: 10.1016/bs.mie.2020.09.004
pii:
doi:
Substances chimiques
MicroRNAs
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109-118Informations de copyright
© 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest statement No conflicts of interest.