X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis.


Journal

Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878

Informations de publication

Date de publication:
01 Jul 2020
Historique:
received: 29 07 2019
accepted: 20 05 2020
entrez: 10 2 2021
pubmed: 11 2 2021
medline: 27 8 2021
Statut: ppublish

Résumé

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting motor neurons. Pre-clinical studies drive the development of animal models that well mimic ALS disorder and enable both the dissection of disease processes and an early assessment of therapy efficacy. A comprehensive knowledge of neuronal and vascular lesions in the brain and spinal cord is an essential factor to understand the development of the disease. Spatial resolution and bidimensional imaging are important drawbacks limiting current neuroimaging tools, while neuropathology relies on protocols that may alter tissue chemistry and structure. In contrast, recent ex vivo studies in mice demonstrated that X-ray phase-contrast tomography enables study of the 3D distribution of both vasculature and neuronal networks, without sample sectioning or use of staining. Here we present our findings on ex vivo SOD1

Identifiants

pubmed: 33566014
pii: S1600577520006785
doi: 10.1107/S1600577520006785
pmc: PMC7336179
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1042-1048

Informations de copyright

open access.

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Auteurs

Ginevra Begani Provinciali (G)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Nicola Pieroni (N)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Inna Bukreeva (I)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Michela Fratini (M)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Lorenzo Massimi (L)

Department of Medical Physics and Biomedical Engineering, University College London, Gower Street, London WC1E 6BT, United Kingdom.

Laura Maugeri (L)

Fondazione Santa Lucia IRCCS, Via Ardeatina 306, 00179 Rome, Italy.

Francesca Palermo (F)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Fabrizio Bardelli (F)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

Alberto Mittone (A)

European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38043 Grenoble, France.

Alberto Bravin (A)

European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38043 Grenoble, France.

Giuseppe Gigli (G)

CNR Nanotec, Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy.

Francesco Gentile (F)

Neuropathology Unit, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Andrea Fossaghi (A)

Neuropathology Unit, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Nilo Riva (N)

Neuropathology Unit, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Angelo Quattrini (A)

Neuropathology Unit, Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Alessia Cedola (A)

Physics Department `Sapienza' University, CNR-Institute of Nanotechnology, Piazzale Aldo Moro 5, 00185 Rome, Italy.

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Classifications MeSH