Expansion of T regulatory lymphocytes by murine bone marrow dendritic cells previously stimulated with Anisakis simplex larval antigens.
Journal
Memorias do Instituto Oswaldo Cruz
ISSN: 1678-8060
Titre abrégé: Mem Inst Oswaldo Cruz
Pays: Brazil
ID NLM: 7502619
Informations de publication
Date de publication:
2021
2021
Historique:
received:
29
10
2020
accepted:
08
01
2021
entrez:
10
2
2021
pubmed:
11
2
2021
medline:
16
2
2021
Statut:
epublish
Résumé
Anisakis simplex antigens present immunomodulatory properties by the induction of tolerogenic dendritic cells (DCs) in mice. To study the capacity of DCs stimulated with A. simplex excretory-secretory (ES) or crude extract (CE) to generate Tregs. To investigate in vitro effects of antigens on the metabolic activity of splenocytes induced by LPS or CpG. Phenotypic and functional characterization of T cells co-cultured with A. simplex-pulsed DCs was performed by flow cytometry. Lymphocyte mitochondrial respiratory activity was estimated by the Alamar Blue® Assay. In C57BL/6J, CD4+CD25-Foxp3+ and CD8+CD25-Foxp3+ populations increased by CE-stimulated-DCs. In BALB/c, CE-stimulated-DCs caused the expansion of CD4+CD25+Foxp3+IL-10+ and CD8+CD25+Foxp3+IL-10+. IFN-γ expression raised in BALB/c CD4+CD25+ and CD4+CD25- for CE and ES, respectively. ES-stimulated-DCs increased CD4+CD25+ Foxp3+ and CD8+CD25- Foxp3+ expression in T cells. The association of ES or CE with LPS produced the increase in splenocyte activity in C57BL/6J. The association of CE with CpG decreased the proliferation caused by CpG in C57BL/6J. A. simplex increase the frequency of Tregs, which in turn produce IL-10 and IFN-γ. The host genetic base is essential in the development of anti-Anisakis immune responses (Th2, Th1, Treg).
Sections du résumé
BACKGROUND
BACKGROUND
Anisakis simplex antigens present immunomodulatory properties by the induction of tolerogenic dendritic cells (DCs) in mice.
OBJECTIVES
OBJECTIVE
To study the capacity of DCs stimulated with A. simplex excretory-secretory (ES) or crude extract (CE) to generate Tregs. To investigate in vitro effects of antigens on the metabolic activity of splenocytes induced by LPS or CpG.
METHODS
METHODS
Phenotypic and functional characterization of T cells co-cultured with A. simplex-pulsed DCs was performed by flow cytometry. Lymphocyte mitochondrial respiratory activity was estimated by the Alamar Blue® Assay.
FINDINGS
RESULTS
In C57BL/6J, CD4+CD25-Foxp3+ and CD8+CD25-Foxp3+ populations increased by CE-stimulated-DCs. In BALB/c, CE-stimulated-DCs caused the expansion of CD4+CD25+Foxp3+IL-10+ and CD8+CD25+Foxp3+IL-10+. IFN-γ expression raised in BALB/c CD4+CD25+ and CD4+CD25- for CE and ES, respectively. ES-stimulated-DCs increased CD4+CD25+ Foxp3+ and CD8+CD25- Foxp3+ expression in T cells. The association of ES or CE with LPS produced the increase in splenocyte activity in C57BL/6J. The association of CE with CpG decreased the proliferation caused by CpG in C57BL/6J.
MAIN CONCLUSIONS
CONCLUSIONS
A. simplex increase the frequency of Tregs, which in turn produce IL-10 and IFN-γ. The host genetic base is essential in the development of anti-Anisakis immune responses (Th2, Th1, Treg).
Identifiants
pubmed: 33566938
pii: S0074-02762021000100300
doi: 10.1590/0074-02760200560
pmc: PMC7874847
pii:
doi:
Substances chimiques
Antigens
0
Forkhead Transcription Factors
0
Interleukin-2 Receptor alpha Subunit
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e200560Références
Immunobiology. 2006;211(5):391-402
pubmed: 16716808
PLoS Pathog. 2011 May;7(5):e1002003
pubmed: 21589896
Infect Immun. 2002 Dec;70(12):6638-45
pubmed: 12438336
J Allergy Clin Immunol. 2016 Sep;138(3):666-675
pubmed: 27476889
J Leukoc Biol. 2009 Dec;86(6):1305-10
pubmed: 19741156
Parasite Immunol. 2005 Oct-Nov;27(10-11):407-15
pubmed: 16179034
J Biomed Res. 2014 Jul;28(4):299-308
pubmed: 25050114
Vet World. 2019 Oct;12(10):1529-1534
pubmed: 31849412
Nat Rev Immunol. 2016 Feb;16(2):102-11
pubmed: 26781939
J Immunol Res. 2015;2015:523875
pubmed: 26114122
Hum Immunol. 2000 Mar;61(3):314-9
pubmed: 10689122
BMC Immunol. 2015 Aug 13;16:44
pubmed: 26268402
Immunity. 2017 Dec 19;47(6):1024-1036
pubmed: 29262347
Exp Parasitol. 1990 Apr;70(3):305-13
pubmed: 2311712
JPEN J Parenter Enteral Nutr. 2016 Feb;40(2):256-63
pubmed: 25403938
J Immunol. 2004 Aug 15;173(4):2699-704
pubmed: 15294988
Biomark Med. 2012 Apr;6(2):151-7
pubmed: 22448789
Cytotechnology. 2016 Aug;68(4):1489-98
pubmed: 26280992
Clin Exp Immunol. 2004 May;136(2):334-40
pubmed: 15086399
Front Immunol. 2019 Mar 21;10:530
pubmed: 30949176
Vet Res. 2012 Mar 22;43:23
pubmed: 22440243
Eur J Immunol. 2010 Feb;40(2):443-8
pubmed: 19941312
Parasite Immunol. 2019 Apr;41(4):e12616
pubmed: 30719721
Scand J Immunol. 2010 Dec;72(6):491-503
pubmed: 21044123
Exp Parasitol. 1983 Jun;55(3):289-98
pubmed: 6852167
Med Hypotheses. 2010 Dec;75(6):623-6
pubmed: 20729003
Curr Opin Allergy Clin Immunol. 2009 Feb;9(1):29-37
pubmed: 19106698
Parasitol Res. 2011 Feb;108(2):477-80
pubmed: 20886233
J Immunol. 1992 Feb 15;148(4):1143-8
pubmed: 1737931
Eur J Immunol. 2007 Jul;37(7):1887-904
pubmed: 17563917
Parasite Immunol. 2018 May;40(5):e12527
pubmed: 29569735
Zentralbl Veterinarmed B. 1998 Dec;45(10):603-10
pubmed: 9916551
PLoS Negl Trop Dis. 2008 Apr 16;2(4):e227
pubmed: 18414649