CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.
Animals
Caenorhabditis elegans Proteins
/ chemistry
Kinesins
/ chemistry
Kinetics
Markov Chains
Microtubule-Associated Proteins
/ chemistry
Microtubules
/ chemistry
Models, Theoretical
Multiprotein Complexes
Protein Binding
Protein Conformation, alpha-Helical
Protein Interaction Domains and Motifs
Spindle Apparatus
/ chemistry
Stochastic Processes
Sus scrofa
Tubulin
/ chemistry
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
10 02 2021
10 02 2021
Historique:
received:
09
05
2019
accepted:
14
01
2021
entrez:
11
2
2021
pubmed:
12
2
2021
medline:
10
8
2021
Statut:
epublish
Résumé
Centralspindlin, a complex of the MKLP1 kinesin-6 and CYK4 GAP subunits, plays key roles in metazoan cytokinesis. CYK4-binding to the long neck region of MKLP1 restricts the configuration of the two MKLP1 motor domains in the centralspindlin. However, it is unclear how the CYK4-binding modulates the interaction of MKLP1 with a microtubule. Here, we performed three-dimensional nanometry of a microbead coated with multiple MKLP1 molecules on a freely suspended microtubule. We found that beads driven by dimeric MKLP1 exhibited persistently left-handed helical trajectories around the microtubule axis, indicating torque generation. By contrast, centralspindlin, like monomeric MKLP1, showed similarly left-handed but less persistent helical movement with occasional rightward movements. Analysis of the fluctuating helical movement indicated that the MKLP1 stochastically makes off-axis motions biased towards the protofilament on the left. CYK4-binding to the neck domains in MKLP1 enables more flexible off-axis motion of centralspindlin, which would help to avoid obstacles along crowded spindle microtubules.
Identifiants
pubmed: 33568771
doi: 10.1038/s42003-021-01704-2
pii: 10.1038/s42003-021-01704-2
pmc: PMC7876049
doi:
Substances chimiques
CYK-4 protein, C elegans
0
Caenorhabditis elegans Proteins
0
MKLP1 protein, C elegans
0
Microtubule-Associated Proteins
0
Multiprotein Complexes
0
Tubulin
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
180Subventions
Organisme : Cancer Research UK
ID : 11985
Pays : United Kingdom
Références
J Cell Sci. 2017 May 15;130(10):1845-1855
pubmed: 28389579
Nature. 1999 Dec 16;402(6763):778-84
pubmed: 10617199
Dev Cell. 2003 Jan;4(1):29-39
pubmed: 12530961
Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):E6582-E6589
pubmed: 27729532
J Cell Sci. 2017 Jun 1;130(11):1904-1916
pubmed: 28420672
Mol Biol Cell. 2003 Oct;14(10):4028-38
pubmed: 14517316
Semin Cell Dev Biol. 2016 May;53:45-56
pubmed: 26964770
Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):10894-10899
pubmed: 28973906
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 Jun;85(6 Pt 1):061916
pubmed: 23005136
Elife. 2019 Feb 13;8:
pubmed: 30758285
Science. 1997 May 9;276(5314):917-22
pubmed: 9139648
Mol Biol Cell. 2007 Dec;18(12):4992-5003
pubmed: 17942600
Elife. 2017 Aug 11;6:
pubmed: 28826477
J Biol Chem. 2013 Jul 5;288(27):19785-95
pubmed: 23720745
Nat Cell Biol. 2009 Jun;11(6):717-23
pubmed: 19430467
Nat Commun. 2020 May 22;11(1):2565
pubmed: 32444784
Protein Expr Purif. 2003 Nov;32(1):83-8
pubmed: 14680943
Annu Rev Cell Dev Biol. 2012;28:29-58
pubmed: 22804577
J Cell Sci. 2006 Jan 1;119(Pt 1):104-14
pubmed: 16352658
J Cell Biol. 1993 Jun;121(5):1083-93
pubmed: 8099076
Mol Biol Cell. 2018 Jul 15;29(13):1743-1752
pubmed: 29771628
Nat Struct Mol Biol. 2008 Oct;15(10):1119-21
pubmed: 18806799
Nat Rev Mol Cell Biol. 2009 Dec;10(12):877-84
pubmed: 19935670
Biophys J. 2015 Apr 21;108(8):2019-27
pubmed: 25902441
Mol Biol Cell. 2002 Jun;13(6):1832-45
pubmed: 12058052
Biophys J. 2008 Feb 1;94(3):L20-2
pubmed: 18083906
PLoS One. 2015 Sep 14;10(9):e0136920
pubmed: 26368807
J Cell Biol. 1998 Mar 23;140(6):1395-405
pubmed: 9508772
Mol Cell. 2012 Apr 27;46(2):147-58
pubmed: 22541555
Proc Natl Acad Sci U S A. 2018 Aug 21;115(34):E7950-E7959
pubmed: 30093386
Nat Commun. 2018 Sep 3;9(1):3571
pubmed: 30177685
J Cell Sci. 2011 Jan 15;124(Pt 2):207-15
pubmed: 21187346
Biophys J. 2019 Jun 18;116(12):2266-2274
pubmed: 31155147
Curr Biol. 2000 Feb 10;10(3):157-60
pubmed: 10679326
Phys Rev E Stat Nonlin Soft Matter Phys. 2014 Feb;89(2):022726
pubmed: 25353527
J Cell Biol. 2009 Sep 7;186(5):727-38
pubmed: 19720876
Nano Lett. 2018 Feb 14;18(2):1290-1295
pubmed: 29380607
Cold Spring Harb Perspect Biol. 2015 Feb 13;7(4):a015834
pubmed: 25680833
Nat Commun. 2017 Apr 10;8:14951
pubmed: 28393873
Curr Biol. 2010 May 25;20(10):927-33
pubmed: 20451386
Biophys J. 2015 Feb 17;108(4):872-879
pubmed: 25692592
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Oct;82(4 Pt 1):041914
pubmed: 21230320
Cytoskeleton (Hoboken). 2020 Sep;77(9):351-361
pubmed: 32845074
PLoS Biol. 2011 Nov;9(11):e1001207
pubmed: 22140358
Structure. 2016 Aug 2;24(8):1322-1334
pubmed: 27452403
Biophys J. 2014 Apr 15;106(8):1691-700
pubmed: 24739168
Biochem Biophys Res Commun. 2010 Oct 8;401(1):53-7
pubmed: 20833139
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Jul;82(1 Pt 1):011917
pubmed: 20866658
Nature. 1990 Oct 25;347(6295):780-2
pubmed: 2146510
Methods Cell Biol. 2017;137:371-385
pubmed: 28065317
Blood. 2013 Jun 6;121(23):4791-9
pubmed: 23570799
Elife. 2014 Jul 28;3:e03205
pubmed: 25069614
Nature. 2006 Feb 16;439(7078):875-8
pubmed: 16382238
Dev Cell. 2002 Jan;2(1):41-54
pubmed: 11782313
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5977-81
pubmed: 11959922
Nat Chem Biol. 2005 Nov;1(6):338-41
pubmed: 16408073
J Mol Biol. 2000 May 12;298(4):663-76
pubmed: 10788328
Curr Biol. 2009 Dec 15;19(23):2043-9
pubmed: 19962307
Biophys J. 2012 Jul 3;103(1):L4-6
pubmed: 22828351
Biophys J. 2018 Nov 20;115(10):1993-2002
pubmed: 30360926
PLoS Biol. 2015 Apr 13;13(4):e1002121
pubmed: 25875822