Computational analyses reveal spatial relationships between nuclear pore complexes and specific lamins.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 04 2021
05 04 2021
Historique:
received:
16
07
2020
revised:
15
10
2020
accepted:
05
01
2021
entrez:
11
2
2021
pubmed:
12
2
2021
medline:
22
9
2021
Statut:
ppublish
Résumé
Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using datasets prepared from subpixel and segmentation analyses of 3D-structured illumination microscopy images of WT and lamin isoform knockout mouse embryo fibroblasts, we determined with high precision the spatial association of NPCs with specific lamin isoform fibers. These relationships are retained in the enlarged lamin meshworks of Lmna-/- and Lmnb1-/- fibroblast nuclei. Cryo-ET observations reveal that the lamin filaments composing the fibers contact the nucleoplasmic ring of NPCs. Knockdown of the ring-associated nucleoporin ELYS induces NPC clusters that exclude lamin A/C fibers but include LB1 and LB2 fibers. Knockdown of the nucleoporin TPR or NUP153 alters the arrangement of lamin fibers and NPCs. Evidence that the number of NPCs is regulated by specific lamin isoforms is presented. Overall the results demonstrate that lamin isoforms and nucleoporins act together to maintain the normal organization of lamin meshworks and NPCs within the nuclear envelope.
Identifiants
pubmed: 33570570
pii: 211786
doi: 10.1083/jcb.202007082
pmc: PMC7883741
pii:
doi:
Substances chimiques
Lamin Type A
0
Lamin Type B
0
Lmna protein, mouse
0
Nuclear Pore Complex Proteins
0
Nup153 protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM106023
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119619
Pays : United States
Organisme : NIH HHS
ID : S10 OD016342
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA080621
Pays : United States
Informations de copyright
© 2021 Kittisopikul et al.
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