A combination of Olea europaea leaf extract and Spirodela polyrhiza extract alleviates atopic dermatitis by modulating immune balance and skin barrier function in a 1-chloro-2,4-dinitrobenzene-induced murine model.
Animals
Cytokines
/ metabolism
Dermatitis, Atopic
/ drug therapy
Dinitrobenzenes
/ chemistry
Disease Models, Animal
Filaggrin Proteins
Immunoglobulin E
/ blood
Intermediate Filament Proteins
/ metabolism
Leukocytes, Mononuclear
/ metabolism
Male
Mice
Olea
/ chemistry
Plant Extracts
/ pharmacology
Skin
/ drug effects
Th2 Cells
/ drug effects
Atopic dermatitis
Flavonoid derivative
Immune balance
Olea europaea
Skin barrier function
Spirodela polyrhiza
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
10
06
2020
revised:
19
10
2020
accepted:
03
11
2020
pubmed:
12
2
2021
medline:
24
4
2021
entrez:
11
2
2021
Statut:
ppublish
Résumé
Atopic dermatitis is a chronic inflammatory skin disease in humans. Although Olea europaea leaf extract (OLE) and Spirodela polyrhiza extract (SPE) have been used to protect against skin damage, the effects of their combined administration on atopic dermatitis have yet to studied. In this study, we evaluated the potential therapeutic effects of an OLE and SPE combination on the progression of atopic dermatitis and the possible mechanisms underlying these effects in 1-chloro-2,4-dinitrobenzene (DNCB)-treated NC/Nga mice. Atopic dermatitis was induced by topical application of 0.2% w/v DNCB prepared in an olive oil:acetone solution (1:3), and thereafter OLE, SPE and OLE + SPE were administered orally for 5 weeks. We determined atopic dermatitis symptoms, serum IgE levels, and levels of cytokine- and gene expression in the dorsal skin and splenocytes, and performed histological and immune cell subtype analyses. The expression of skin barrier-related proteins (filaggrin, sirtuin 1, and claudin 1) was also evaluated. The OLE + SPE combination significantly ameliorated atopic dermatitis symptoms, including dermatitis scores, and reduced epidermal thickness and infiltration of different inflammatory cells in mice with DNCB-induced atopic dermatitis. It also significantly reduced the number of CD4 Taken together, our findings indicate that administration of a combination of OLE and SPE can alleviate atopic dermatitis symptoms by regulating immune balance and skin barrier function and may be an effective therapeutic option for the treatment of atopic dermatitis.
Sections du résumé
BACKGROUND
BACKGROUND
Atopic dermatitis is a chronic inflammatory skin disease in humans. Although Olea europaea leaf extract (OLE) and Spirodela polyrhiza extract (SPE) have been used to protect against skin damage, the effects of their combined administration on atopic dermatitis have yet to studied.
PURPOSE
OBJECTIVE
In this study, we evaluated the potential therapeutic effects of an OLE and SPE combination on the progression of atopic dermatitis and the possible mechanisms underlying these effects in 1-chloro-2,4-dinitrobenzene (DNCB)-treated NC/Nga mice.
METHODS
METHODS
Atopic dermatitis was induced by topical application of 0.2% w/v DNCB prepared in an olive oil:acetone solution (1:3), and thereafter OLE, SPE and OLE + SPE were administered orally for 5 weeks. We determined atopic dermatitis symptoms, serum IgE levels, and levels of cytokine- and gene expression in the dorsal skin and splenocytes, and performed histological and immune cell subtype analyses. The expression of skin barrier-related proteins (filaggrin, sirtuin 1, and claudin 1) was also evaluated.
RESULTS
RESULTS
The OLE + SPE combination significantly ameliorated atopic dermatitis symptoms, including dermatitis scores, and reduced epidermal thickness and infiltration of different inflammatory cells in mice with DNCB-induced atopic dermatitis. It also significantly reduced the number of CD4
CONCLUSION
CONCLUSIONS
Taken together, our findings indicate that administration of a combination of OLE and SPE can alleviate atopic dermatitis symptoms by regulating immune balance and skin barrier function and may be an effective therapeutic option for the treatment of atopic dermatitis.
Identifiants
pubmed: 33571899
pii: S0944-7113(20)30238-5
doi: 10.1016/j.phymed.2020.153407
pii:
doi:
Substances chimiques
2,4-dinitrobenzene
0
Cytokines
0
Dinitrobenzenes
0
FLG protein, human
0
Filaggrin Proteins
0
Intermediate Filament Proteins
0
Plant Extracts
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
153407Informations de copyright
Copyright © 2020 Elsevier GmbH. All rights reserved.