PHi-C: deciphering Hi-C data into polymer dynamics.


Journal

NAR genomics and bioinformatics
ISSN: 2631-9268
Titre abrégé: NAR Genom Bioinform
Pays: England
ID NLM: 101756213

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 18 07 2019
revised: 26 02 2020
accepted: 13 03 2020
entrez: 12 2 2021
pubmed: 13 2 2021
medline: 13 2 2021
Statut: epublish

Résumé

Genomes are spatiotemporally organized within the cell nucleus. Genome-wide chromosome conformation capture (Hi-C) technologies have uncovered the 3D genome organization. Furthermore, live-cell imaging experiments have revealed that genomes are functional in 4D. Although computational modeling methods can convert 2D Hi-C data into population-averaged static 3D genome models, exploring 4D genome nature based on 2D Hi-C data remains lacking. Here, we describe a 4D simulation method, PHi-C (polymer dynamics deciphered from Hi-C data), that depicts 4D genome features from 2D Hi-C data by polymer modeling. PHi-C allows users to interpret 2D Hi-C data as physical interaction parameters within single chromosomes. The physical interaction parameters can then be used in the simulations and analyses to demonstrate dynamic characteristics of genomic loci and chromosomes as observed in live-cell imaging experiments. PHi-C is available at https://github.com/soyashinkai/PHi-C.

Identifiants

pubmed: 33575580
doi: 10.1093/nargab/lqaa020
pii: lqaa020
pmc: PMC7671433
doi:

Types de publication

Journal Article

Langues

eng

Pagination

lqaa020

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.

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Auteurs

Soya Shinkai (S)

Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan.
Research Center for the Mathematics on Chromatin Live Dynamics, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.

Masaki Nakagawa (M)

Research Center for the Mathematics on Chromatin Live Dynamics, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Graduate School of Information Science and Technology, Osaka University, Suita 565-0871, Japan.

Takeshi Sugawara (T)

Research Center for the Mathematics on Chromatin Live Dynamics, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Yuichi Togashi (Y)

Research Center for the Mathematics on Chromatin Live Dynamics, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Laboratory for Cell Field Structure, RIKEN Center for Biosystems Dynamics Research, Higashi-Hiroshima 739-0046, Japan.

Hiroshi Ochiai (H)

Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
PRESTO, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan.

Ryuichiro Nakato (R)

Institute for Quantitative Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

Yuichi Taniguchi (Y)

PRESTO, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan.
Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, Suita 565-0874, Japan.

Shuichi Onami (S)

Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan.

Classifications MeSH