Nanoscale Assembly of Functional Peptides with Divergent Programming Elements.

amyloid chirality d-amino acids hydrogels peptide proline self-assembly

Journal

ACS nano
ISSN: 1936-086X
Titre abrégé: ACS Nano
Pays: United States
ID NLM: 101313589

Informations de publication

Date de publication:
23 02 2021
Historique:
pubmed: 13 2 2021
medline: 15 5 2021
entrez: 12 2 2021
Statut: ppublish

Résumé

Self-assembling peptides are being applied both in the biomedical area and as building blocks in nanotechnology. Their applications are closely linked to their modes of self-assembly, which determine the functional nanostructures that they form. This work brings together two structural elements that direct nanoscale self-association in divergent directions: proline as a β-breaker and the β-structure-associated diphenylalanine motif, into a single tripeptide sequence. Amino acid chirality was found to resolve the tension inherent to these conflicting self-assembly instructions. Stereoconfiguration determined the ability of each of the eight possible Pro-Phe-Phe stereoisomers to self-associate into diverse nanostructures, including nanoparticles, nanotapes, or fibrils, which yielded hydrogels with gel-to-sol transition at a physiologically relevant temperature. Three single-crystal structures and all-atom molecular dynamics simulations elucidated the ability of each peptide to establish key interactions to form long-range assemblies (

Identifiants

pubmed: 33576622
doi: 10.1021/acsnano.0c09386
pmc: PMC8023796
doi:

Substances chimiques

Amyloid 0
Hydrogels 0
Peptides 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3015-3025

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Auteurs

Ana M Garcia (AM)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Michele Melchionna (M)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.
INSTM, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Ottavia Bellotto (O)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Slavko Kralj (S)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.
Materials Synthesis Department, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.

Sabrina Semeraro (S)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Evelina Parisi (E)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Daniel Iglesias (D)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Paola D'Andrea (P)

Life Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Rita De Zorzi (R)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

Attilio V Vargiu (AV)

Physics Department, University of Cagliari, S.P. 8, km. 0.700, 09042 Monserrato, Italy.

Silvia Marchesan (S)

Chemical and Pharmaceutical Sciences Department, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.
INSTM, University of Trieste, Via Giorgieri 1, 34127 Trieste, Italy.

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Classifications MeSH