Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection.
Animals
Antibodies, Neutralizing
B-Lymphocytes
/ immunology
COVID-19
/ immunology
COVID-19 Vaccines
/ administration & dosage
Macaca fascicularis
Mice
Mice, Inbred BALB C
Models, Animal
Nanoparticles
/ administration & dosage
Rabbits
SARS-CoV-2
/ physiology
Spike Glycoprotein, Coronavirus
/ blood
T-Lymphocytes
/ immunology
Viral Load
B cells
COVID-19
SARS-CoV-2
antibodies
immunity
macaques
nanoparticles
protection
vaccine
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
04 03 2021
04 03 2021
Historique:
received:
03
11
2020
revised:
23
12
2020
accepted:
21
01
2021
pubmed:
13
2
2021
medline:
12
3
2021
entrez:
12
2
2021
Statut:
ppublish
Résumé
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits, and cynomolgus macaques. The vaccine-induced immunity protects macaques against a high-dose challenge, resulting in strongly reduced viral infection and replication in the upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic.
Identifiants
pubmed: 33577765
pii: S0092-8674(21)00078-7
doi: 10.1016/j.cell.2021.01.035
pmc: PMC7834972
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
COVID-19 Vaccines
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1188-1200.e19Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests N.P.K. is a co-founder, shareholder, and chair of the scientific advisory board of Icosavax. The remaining authors declare no competing interests. Amsterdam UMC has filed a patent application concerning the SARS-CoV-2 mAbs used here (Brouwer et al., 2020). N.P.K. has a nonprovisional US patent (no. 14/930,792) related to I53-50 (Bale et al., 2016).
Références
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357
pubmed: 28807998
Science. 2020 Sep 18;369(6510):1501-1505
pubmed: 32703906
Science. 2019 Feb 8;363(6427):649-654
pubmed: 30573546
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242
Retrovirology. 2015 Sep 26;12:82
pubmed: 26410741
Radiology. 2020 Jun;295(3):715-721
pubmed: 32053470
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Nat Nanotechnol. 2020 Aug;15(8):716-723
pubmed: 32601450
N Engl J Med. 2012 Dec 13;367(24):2284-95
pubmed: 23136909
Nat Commun. 2020 Aug 21;11(1):4207
pubmed: 32826924
Nature. 2020 Oct;586(7830):578-582
pubmed: 32731258
N Engl J Med. 2020 Oct 15;383(16):1544-1555
pubmed: 32722908
Cell. 2020 Nov 25;183(5):1367-1382.e17
pubmed: 33160446
Cell. 2020 Jul 9;182(1):73-84.e16
pubmed: 32425270
J Exp Med. 2020 Nov 2;217(11):
pubmed: 32692348
J Struct Biol. 2009 Apr;166(1):95-102
pubmed: 19263523
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Sci Adv. 2021 Mar 19;7(12):
pubmed: 33608249
Vaccine. 2020 Nov 25;38(50):7892-7896
pubmed: 33139139
J Clin Microbiol. 2020 Oct 21;58(11):
pubmed: 32826322
Ultramicroscopy. 1999 Jul;77(3-4):153-61
pubmed: 10406132
Vaccine. 2018 Jan 2;36(1):148-154
pubmed: 29174683
Sci Adv. 2021 Mar 19;7(12):
pubmed: 33741598
ACS Cent Sci. 2021 Jan 27;7(1):183-199
pubmed: 33527087
J Exp Med. 2017 Jul 3;214(7):2139-2152
pubmed: 28637884
J Virol. 1998 Nov;72(11):8463-71
pubmed: 9765382
PLoS Pathog. 2020 Aug 11;16(8):e1008665
pubmed: 32780770
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Science. 2016 Jul 22;353(6297):389-94
pubmed: 27463675
Science. 2020 Jul 3;369(6499):77-81
pubmed: 32376603
Mol Ther. 2006 Dec;14(6):883-92
pubmed: 16959541
J Virol. 2002 Sep;76(17):8875-89
pubmed: 12163607
Nat Commun. 2021 Jan 11;12(1):244
pubmed: 33431842
Nat Commun. 2019 Oct 11;10(1):4660
pubmed: 31604920
Comput Struct Biotechnol J. 2015 Nov 26;14:58-68
pubmed: 26862374
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Cell. 2019 Mar 7;176(6):1420-1431.e17
pubmed: 30849373
Lancet Infect Dis. 2020 Apr;20(4):425-434
pubmed: 32105637
Nat Commun. 2021 Mar 1;12(1):1346
pubmed: 33649323
Cell Rep Med. 2021 Oct 19;2(10):100420
pubmed: 34604818
J Clin Invest. 2019 Jul 2;129(8):3185-3200
pubmed: 31264971
Genome Res. 2017 May;27(5):722-736
pubmed: 28298431
Nat Commun. 2019 Sep 19;10(1):4272
pubmed: 31537780
Cell. 2020 Oct 29;183(3):730-738.e13
pubmed: 32979942
Science. 2020 Aug 21;369(6506):956-963
pubmed: 32540903
Expert Rev Vaccines. 2019 Mar;18(3):269-280
pubmed: 30707635
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734
pubmed: 32376634
Nature. 2021 Mar;591(7851):639-644
pubmed: 33461210
J Clin Invest. 2016 Mar 1;126(3):799-808
pubmed: 26928033
Cell. 2020 Aug 6;182(3):713-721.e9
pubmed: 32778225
Nat Commun. 2020 Mar 13;11(1):1334
pubmed: 32170079
Nature. 2020 Oct;586(7830):583-588
pubmed: 32731257
Immunity. 2020 Dec 15;53(6):1315-1330.e9
pubmed: 33275896
Cell. 2020 Oct 1;183(1):169-184.e13
pubmed: 32931734
Nat Commun. 2015 Sep 03;6:8143
pubmed: 26333350
Nat Commun. 2019 May 29;10(1):2355
pubmed: 31142746
Science. 2014 Dec 12;346(6215):1380-1383
pubmed: 25504724
Nature. 2020 Sep;585(7826):584-587
pubmed: 32698191
Lancet Infect Dis. 2020 Jun;20(6):697-706
pubmed: 32224310
mBio. 2021 Mar 2;12(2):
pubmed: 33653892
Emerg Infect Dis. 2020 Jul;26(7):1478-1488
pubmed: 32267220
Science. 2020 Aug 14;369(6505):806-811
pubmed: 32434945
Science. 2020 Jul 17;369(6501):330-333
pubmed: 32366695
ACS Nano. 2018 Sep 25;12(9):8855-8866
pubmed: 30028591
Genome Res. 2012 Mar;22(3):568-76
pubmed: 22300766
Nature. 2013 Jul 4;499(7456):102-6
pubmed: 23698367
Nat Rev Immunol. 2010 Nov;10(11):787-96
pubmed: 20948547
Cell. 2015 Dec 17;163(7):1702-15
pubmed: 26687358
Science. 2020 Aug 7;369(6504):643-650
pubmed: 32540902
Nat Med. 2020 May;26(5):681-687
pubmed: 32327758
Nat Commun. 2021 Jan 14;12(1):372
pubmed: 33446655
Protein Sci. 2018 Jan;27(1):14-25
pubmed: 28710774