Development of cycling probe based real-time PCR methodology for influenza A viruses possessing the PA/I38T amino acid substitution associated with reduced baloxavir susceptibility.

Amino Acid Substitution Animals Antiviral Agents / pharmacology Cell Line Dibenzothiepins / pharmacology Humans Influenza A virus / drug effects Microbial Sensitivity Tests Morpholines / pharmacology Pyridones / pharmacology RNA, Viral / biosynthesis RNA-Dependent RNA Polymerase / genetics Real-Time Polymerase Chain Reaction / methods Triazines / pharmacology Viral Proteins / genetics Virus Replication / drug effects
Antiviral susceptibility Baloxavir marboxil Cycling probe real-time PCR Influenza virus PA/I38T substitution

Journal

Antiviral research
ISSN: 1872-9096
Titre abrégé: Antiviral Res
Pays: Netherlands
ID NLM: 8109699

Informations de publication

Date de publication:
04 2021
Historique:
received: 15 05 2020
revised: 04 02 2021
accepted: 06 02 2021
pubmed: 13 2 2021
medline: 13 10 2021
entrez: 12 2 2021
Statut: ppublish

Résumé

Baloxavir marboxil has been used for influenza treatment since March 2018 in Japan. After baloxavir treatment, the most frequently detected substitution is Ile38Thr in polymerase acidic protein (PA/I38T), and this substitution reduces baloxavir susceptibility in influenza A viruses. To rapidly investigate the frequency of PA/I38T in influenza A (H1N1)pdm09 and A (H3N2) viruses in clinical samples, we established a rapid real-time system to detect single nucleotide polymorphisms in PA, using cycling probe real-time PCR. We designed two sets of probes that were labeled with either 6-carboxyfluorescein (FAM) or 6-carboxy-X-rhodamine (ROX) to identify PA/I38 (wild type strain) or PA/I38T, respectively. The established cycling probe real-time PCR system showed a dynamic linear range of 10

Identifiants

DOI: 10.1016/j.antiviral.2021.105036 PMID: 33577807
pubmed: 33577807
pii: S0166-3542(21)00026-7
doi: 10.1016/j.antiviral.2021.105036
pii:
doi:

Substances chimiques

Antiviral Agents 0
Dibenzothiepins 0
Morpholines 0
PA protein, influenza viruses 0
Pyridones 0
RNA, Viral 0
Triazines 0
Viral Proteins 0
baloxavir 4G86Y4JT3F
RNA-Dependent RNA Polymerase EC 2.7.7.48

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105036

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Hidekazu Osada (H)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan; Infectious Diseases Research Center of Niigata University in Myanmar, Yangon, Yangon Region, Myanmar. Electronic address: hosada@med.niigata-u.ac.jp.

Irina Chon (I)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan.

Wint Wint Phyu (WW)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan.

Keita Wagatsuma (K)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan.

Nobuo Nagata (N)

Hiraoka-Kohen Pediatric Clinic, Sapporo City, Japan.

Takashi Kawashima (T)

Kawashima Internal Medicine Clinic, Shibukawa City, Japan.

Isamu Sato (I)

Yoiko Pediatric Clinic, Niigata City, Japan.

Tadashi Saito (T)

Pediatric Department, Tako Central Hospital, Tako Town, Japan.

Naoki Kodo (N)

Kodo Pediatric Clinic, Uji City, Japan.

Hironori Masaki (H)

Masaki Respiratory Medicine Clinic, Nagasaki City, Japan.

Norichika Asoh (N)

Juzenkai Hospital, Nagasaki City, Japan.

Yoshiko Tsuchihashi (Y)

Juzenkai Hospital, Nagasaki City, Japan.

Yutaka Shirahige (Y)

Shirahige Clinic, Nagasaki City, Japan.

Yasuhiko Ono (Y)

Ono Pediatric Clinic, Isahaya City, Japan.

Yasushi Shimada (Y)

Shimada Pediatric Clinic, Kamiamakusa City, Japan.

Hirotsune Hamabata (H)

Awase Daiichi Clinic, Okinawa City, Japan.

Kousuke Saito (K)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan.

Reiko Saito (R)

Division of International Health (Public Health), Graduate School of Medical and Dental Sciences, Niigata University, Niigata City, Japan; Infectious Diseases Research Center of Niigata University in Myanmar, Yangon, Yangon Region, Myanmar.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Substitution d'acide aminé › Development of cycling probe based real-time...
questionsmedicales.fr › Eucaryotes › Animaux › Development of cycling probe based real-time...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antiviraux › Development of cycling probe based real-time...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Development of cycling probe based real-time...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques 3 noyaux › Dibenzothiépines › Development of cycling probe based real-time...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Development of cycling probe based real-time...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Orthomyxoviridae › Influenzavirus A › Virus de la grippe A › Development of cycling probe based real-time...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Development of cycling probe based real-time...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Oxazines › Morpholines › Development of cycling probe based real-time...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyridines › Pyridones › Development of cycling probe based real-time...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Development of cycling probe based real-time...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › RNA replicase › Development of cycling probe based real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Development of cycling probe based real-time...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Triazines › Development of cycling probe based real-time...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Development of cycling probe based real-time...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Development of cycling probe based real-time...